Kulakowska M, Lugowski C, Romanowska E, Bundle DR The comparison of monoclonal antibodies specific for two general epitopes located on Citrobacter O36 lipopolysaccharide Carbohydrate Research178 (1988)
233-241
Monoclonal antibodies have been generated to Citrobacter 036 by the hybridoma technique and selected for lipopolysaccharide binding. The quantitative specificity of four clonotypes were analyzed by precipitin-inhibition assay, immunoblotting, and affinity electrophoresis. The 036-specific polysaccharide and core oligosaccharide fractions were used as inhibitors of the precipitation of these antibodies by the homologous lipopolysaccharide. Association constants of monomers of the monoclonal antibodies were determined by affinity electrophoresis. Association constants measured with lipopolysaccharide ranged from 8.3 × 102 to 1.7 × 104, and with the O-specific polysaccharide from 8.7 × 102 to 3.7 × 103. The results obtained by these three methods are in full accordance with the hypothesis that the monoclonal antibodies examined were raised to two general epitopes of Citrobacter 036 lipopolysaccharide. These are the O-specific polysaccharide and an O-acetylated structure that includes the O-specific oligosaccharide-core region.
Comments, role: partial structure, additional L-glycero-D-manno-heptose and diphosphoetanolamine groups are attached to the hepotose region at unknown positions
Related record ID(s): 115400, 115401 NCBI Taxonomy refs (TaxIDs):544 Reference(s) to other database(s): GlycomeDB:37949 Show glycosyltransferases
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