Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Citrobacter [ICD11:
XN0FZ 
]
The structure was elucidated in this paperPublication DOI: 10.1016/0008-6215(88)84131-4Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 Czerska, 53-114 Wrocław Poland, Division of Biological Sciences, National Research Council of Canada, Ottawa K1A 0R6 Canada
The structure of Citrobacter O23-specific polysaccharide has been shown by sugar and methylation analyses of the native and chemically degraded polysaccharide and by 1H- and 13C-n.m.r. spectroscopy to consist of the tetrasaccharide repeating-units: [structure: see text], 80% of which are substituted by O-acetyl groups.
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_130701,IEDB_136104,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_140116,IEDB_141584,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_885822,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GLC-MS, gel filtration, NMR-2D, sugar analysis, TLC, GLC, paper chromatography, optical rotation measurement, de-N-acylation/deamination
Comments, role: Citrobacter sp. O23 strain 87/57; O-Deacetylated OPS; in native OPS 80 % repeating units are substituted by O-acetyl groups in unknown positions.
Related record ID(s): 9250, 22712, 25631, 108631
NCBI Taxonomy refs (TaxIDs): 544Reference(s) to other database(s): GTC:G82509QD, GlycomeDB:
3519
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2,2 aDManp 102.0 71.2-71.7 70.2-70.4 73.7 71.7-72.2 60.1-61.1
3,2 aDManp 99.7 78.2 70.2-70.4 67.0 73.7 60.1-61.1
3 bDManp 101.5 77.0 71.2-71.7 66.3 76.6 60.1-61.1
2 Ac
aDGalpN 98.4 49.2 75.9 68.6 71.7-72.2 60.1-61.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2,2 aDManp 5.05 ? ? ? ? ?
3,2 aDManp 5.33 4.11 4.04 ? ? ?
3 bDManp 4.80 3.99 3.74 3.60 3.41 3.73-3.95
2 Ac
aDGalpN 5.31 4.29 4.00 4.16 ? ?
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2,2 aDManp 102.0/5.05 71.2-71.7/? 70.2-70.4/? 73.7/? 71.7-72.2/? 60.1-61.1/?
3,2 aDManp 99.7/5.33 78.2/4.11 70.2-70.4/4.04 67.0/? 73.7/? 60.1-61.1/?
3 bDManp 101.5/4.80 77.0/3.99 71.2-71.7/3.74 66.3/3.60 76.6/3.41 60.1-61.1/3.73-3.95
2 Ac
aDGalpN 98.4/5.31 49.2/4.29 75.9/4.00 68.6/4.16 71.7-72.2/? 60.1-61.1/?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2,2 | aDManp | 5.05 | ? | ? | ? | ? | ? |
| 3,2 | aDManp | 5.33 | 4.11 | 4.04 | ? | ? | ? |
| 3 | bDManp | 4.80 | 3.99 | 3.74 | 3.60 | 3.41 | 3.73
3.95 |
| 2 | Ac | |
| | aDGalpN | 5.31 | 4.29 | 4.00 | 4.16 | ? | ? |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2,2 | aDManp | 102.0 | 71.2
71.7 | 70.2
70.4 | 73.7 | 71.7
72.2 | 60.1
61.1 |
| 3,2 | aDManp | 99.7 | 78.2 | 70.2
70.4 | 67.0 | 73.7 | 60.1
61.1 |
| 3 | bDManp | 101.5 | 77.0 | 71.2
71.7 | 66.3 | 76.6 | 60.1
61.1 |
| 2 | Ac | |
| | aDGalpN | 98.4 | 49.2 | 75.9 | 68.6 | 71.7
72.2 | 60.1
61.1 |
|
There is only one chemically distinct structure:
Found 
report error