Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Sus scrofa
Associated disease: pleuropneumonia [ICD11:
CA40 
, ICD11:
CA40.Y ]
The structure was elucidated in this paperPublication DOI: 10.1016/0008-6215(88)84084-9Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Division of Biological Sciences, National Research Council, Ottawa K1A OR6 Canada
The capsular polymer of Actinobacillus pleuropneumoniae serotype 6 (ATCC 33590) was investigated by methylation, dephosphorylation, partial hydrolysis, and 1H- and 13C-n.m.r. methods. It was found to be a high-molecular-weight polymer composed of triglycosylglycerol phosphate repeating-units joined through monophosphate diester linkages, and has the structure: [formula; see text].
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p. 330
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_151531,IEDB_885822
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, gel filtration, NMR-2D, sugar analysis, ion-exchange chromatography, HF treatment, optical rotation measurement
Comments, role: Parent molecule: teichoate
NCBI Taxonomy refs (TaxIDs): 754344Reference(s) to other database(s): GTC:G48815HM
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
0,1,3,2 Ac
0,1,3 aDGlcpN 94.12 54.03 71.78 70.16 72.46 65.02
0,1,2 Ac
0,1 bDGalpN 102.10 51.48 75.85 64.42 75.85 61.90
0,2,2 Ac
0,2 aDGalpN 97.47 50.60 68.89 69.51 71.96 62.23
0 x?Gro 70.44 75.66 65.02
P
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
0,1,3,2 Ac
0,1,3 aDGlcpN 5.04 3.98 3.62 3.55 3.67 4.05-4.09
0,1,2 Ac
0,1 bDGalpN 4.57 4.08 3.79 4.11 3.66 3.76-3.81
0,2,2 Ac
0,2 aDGalpN 5.11 4.22 3.91 3.98 4.13 3.76-3.80
0 x?Gro 3.81-4.13 4.06 3.93-4.02
P
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
0,1,3,2 Ac
0,1,3 aDGlcpN 94.12/5.04 54.03/3.98 71.78/3.62 70.16/3.55 72.46/3.67 65.02/4.05-4.09
0,1,2 Ac
0,1 bDGalpN 102.10/4.57 51.48/4.08 75.85/3.79 64.42/4.11 75.85/3.66 61.90/3.76-3.81
0,2,2 Ac
0,2 aDGalpN 97.47/5.11 50.60/4.22 68.89/3.91 69.51/3.98 71.96/4.13 62.23/3.76-3.80
0 x?Gro 70.44/3.81-4.13 75.66/4.06 65.02/3.93-4.02
P
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 0,1,3,2 | Ac | |
| 0,1,3 | aDGlcpN | 5.04 | 3.98 | 3.62 | 3.55 | 3.67 | 4.05
4.09 |
| 0,1,2 | Ac | |
| 0,1 | bDGalpN | 4.57 | 4.08 | 3.79 | 4.11 | 3.66 | 3.76
3.81 |
| 0,2,2 | Ac | |
| 0,2 | aDGalpN | 5.11 | 4.22 | 3.91 | 3.98 | 4.13 | 3.76
3.80 |
| 0 | x?Gro | 3.81
4.13 | 4.06 | 3.93
4.02 | |
| | P | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 0,1,3,2 | Ac | |
| 0,1,3 | aDGlcpN | 94.12 | 54.03 | 71.78 | 70.16 | 72.46 | 65.02 |
| 0,1,2 | Ac | |
| 0,1 | bDGalpN | 102.10 | 51.48 | 75.85 | 64.42 | 75.85 | 61.90 |
| 0,2,2 | Ac | |
| 0,2 | aDGalpN | 97.47 | 50.60 | 68.89 | 69.51 | 71.96 | 62.23 |
| 0 | x?Gro | 70.44 | 75.66 | 65.02 | |
| | P | |
|
There is only one chemically distinct structure:
Found 
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(previously named: Haemophilus pleuropneumoniae 6 (ATCC 33590))