The structure of the capsular antigen from Escherichia coli O8:K46:H30 was elucidated by methylation analysis and 1D and 2D 1H- and 13C-NMR spectroscopy, and by methylation analysis, 1D- and 2D-NMR spectroscopy, and FABMS of the oligosaccharide-alditol obtained after dephosphorylation of the polymer with aqueous hydrofluoric acid. The capsular polymer is of the teichoic acid type and has the following repeating unit. [Formula: see text].
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
0,2,3,3,2 bDGlcp 102.86 74.54 76.92 70.47 76.81 61.78
0,2,3,3 bDGalp 101.55 77.52 78.55 68.68 75.63 61.33
0,2,3,2 Ac 175.02 23.41
0,2,3 bDGlcpN 103.73 55.34 82.14 68.92 75.90 61.65
0,2 aLRhap 99.71 70.81 80.97 71.80 69.91 17.59
0 x?Gro 65.83 76.23 60.62
P
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
0,2,3,3,2 bDGlcp 4.790 3.247 3.500 3.380 3.430 3.737-3.907
0,2,3,3 bDGalp 4.660 3.928 4.312 4.123 3.738 3.830-3.920
0,2,3,2 Ac - 2.05
0,2,3 bDGlcpN 4.750 3.886 3.923 3.590 3.490 3.775-3.775
0,2 aLRhap 4.970 4.220 3.875 3.487 3.911 1.298
0 x?Gro 4.050-4.050 3.927 3.741-3.741
P
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
0,2,3,3,2 bDGlcp 102.86/4.790 74.54/3.247 76.92/3.500 70.47/3.380 76.81/3.430 61.78/3.737-3.907
0,2,3,3 bDGalp 101.55/4.660 77.52/3.928 78.55/4.312 68.68/4.123 75.63/3.738 61.33/3.830-3.920
0,2,3,2 Ac 23.41/2.05
0,2,3 bDGlcpN 103.73/4.750 55.34/3.886 82.14/3.923 68.92/3.590 75.90/3.490 61.65/3.775-3.775
0,2 aLRhap 99.71/4.970 70.81/4.220 80.97/3.875 71.80/3.487 69.91/3.911 17.59/1.298
0 x?Gro 65.83/4.050-4.050 76.23/3.927 60.62/3.741-3.741
P
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