Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperPublication DOI: 10.1016/0008-6215(92)80088-IJournal NLM ID: 0043535Publisher: Elsevier
Institutions: School of Pharmaceutical Sciences, Rhodes University, Grahamstown 6140 South Africa
The structure of the capsular antigen of E. coli O8:K102:H− was investigated by methylation analysis, β-elimination of the methylated polys
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.206, structure 3 (PS)
Compound class: CPS, K-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_423153,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_31,SB_62,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, Li/ethylenediamine degradation
Comments, role: E. coli 08:K102:H- (No. 6CBlO/l)
Related record ID(s): 2725
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G35638LL, GlycomeDB:
6294
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,4 bDGalp 103.74 71.40 82.84 69.27 75.26 ?
4,3,4 aDGlcp 99.86 72.36 73.54 69.84 73.04 61.60
4,3 bDGlcpA 105.07 73.96 76.47 77.82 75.19 ?
4 aDGalp 100.99 68.57 80.85 77.17 70.80 60.79
bDGalp 105.20 71.95 72.97 78.08 75.89 ?
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,4 bDGalp 4.85 3.75 3.88 4.15 3.68 ?
4,3,4 aDGlcp 5.43 3.55 3.67 3.46 3.60 3.80-3.80
4,3 bDGlcpA 4.74 3.52 3.83 3.91 4.11 -
4 aDGalp 5.01 4.16 4.13 4.44 4.41 3.64-3.78
bDGalp 4.74 3.67 3.77 4.05 3.78 ?
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,4 bDGalp 103.74/4.85 71.40/3.75 82.84/3.88 69.27/4.15 75.26/3.68 ?/?
4,3,4 aDGlcp 99.86/5.43 72.36/3.55 73.54/3.67 69.84/3.46 73.04/3.60 61.60/3.80-3.80
4,3 bDGlcpA 105.07/4.74 73.96/3.52 76.47/3.83 77.82/3.91 75.19/4.11
4 aDGalp 100.99/5.01 68.57/4.16 80.85/4.13 77.17/4.44 70.80/4.41 60.79/3.64-3.78
bDGalp 105.20/4.74 71.95/3.67 72.97/3.77 78.08/4.05 75.89/3.78 ?/?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,4 | bDGalp | 4.85 | 3.75 | 3.88 | 4.15 | 3.68 | ? |
| 4,3,4 | aDGlcp | 5.43 | 3.55 | 3.67 | 3.46 | 3.60 | 3.80
3.80 |
| 4,3 | bDGlcpA | 4.74 | 3.52 | 3.83 | 3.91 | 4.11 |
|
| 4 | aDGalp | 5.01 | 4.16 | 4.13 | 4.44 | 4.41 | 3.64
3.78 |
| | bDGalp | 4.74 | 3.67 | 3.77 | 4.05 | 3.78 | ? |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,4 | bDGalp | 103.74 | 71.40 | 82.84 | 69.27 | 75.26 | ? |
| 4,3,4 | aDGlcp | 99.86 | 72.36 | 73.54 | 69.84 | 73.04 | 61.60 |
| 4,3 | bDGlcpA | 105.07 | 73.96 | 76.47 | 77.82 | 75.19 | ? |
| 4 | aDGalp | 100.99 | 68.57 | 80.85 | 77.17 | 70.80 | 60.79 |
| | bDGalp | 105.20 | 71.95 | 72.97 | 78.08 | 75.89 | ? |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":103.74,"l":"1","c":[0,0,225]},{"s":71.4,"l":"2","c":[0,0,225]},{"s":82.84,"l":"3","c":[0,0,225]},{"s":69.27,"l":"4","c":[0,0,225]},{"s":75.26,"l":"5","c":[0,0,225]},{"s":99.86,"l":"1","c":[230,0,30]},{"s":72.36,"l":"2","c":[230,0,30]},{"s":73.54,"l":"3","c":[230,0,30]},{"s":69.84,"l":"4","c":[230,0,30]},{"s":73.04,"l":"5","c":[230,0,30]},{"s":61.6,"l":"6","c":[230,0,30]},{"s":105.07,"l":"1","c":[0,165,0]},{"s":73.96,"l":"2","c":[0,165,0]},{"s":76.47,"l":"3","c":[0,165,0]},{"s":77.82,"l":"4","c":[0,165,0]},{"s":75.19,"l":"5","c":[0,165,0]},{"s":100.99,"l":"1","c":[120,0,225]},{"s":68.57,"l":"2","c":[120,0,225]},{"s":80.85,"l":"3","c":[120,0,225]},{"s":77.17,"l":"4","c":[120,0,225]},{"s":70.8,"l":"5","c":[120,0,225]},{"s":60.79,"l":"6","c":[120,0,225]},{"s":105.2,"l":"1","c":[0,192,255]},{"s":71.95,"l":"2","c":[0,192,255]},{"s":72.97,"l":"3","c":[0,192,255]},{"s":78.08,"l":"4","c":[0,192,255]},{"s":75.89,"l":"5","c":[0,192,255]}])
The spectrum also has 3 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Found 
report error