Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
 
Publication DOI: 10.1371/journal.pone.0155115
Journal NLM ID: 101285081
Publisher: San Francisco, CA: Public Library of Science
Correspondence: wangleinankai.edu.cn
Institutions: Department of Biotechnology and Molecular Biology, University of Opole, Opole, Poland, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, P. R. China, Tianjin Research Center for Functional Genomics and Biochips, TEDA College, Nankai University, Tianjin, P. R. China, Tianjin Key Laboratory of Microbial Functional Genomics, TEDA College, Nankai University, Tianjin, P. R. China, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University, Tianjin, P. R. China

Hafnia alvei is a facultative and rod-shaped gram-negative bacterium that belongs to the Enterobacteriaceae family. Although it has been more than 50 years since the genus was identified, very little is known about variations among Hafnia species. Diversity in O-antigens (O-polysaccharide, OPS) is thought to be a major factor in bacterial adaptation to different hosts and situations and variability in the environment. Antigenic variation is also an important factor in pathogenicity that has been used to define clones within a number of species. The genes that are required to synthesize OPS are always clustered within the bacterial chromosome. A serotyping scheme including 39 O-serotypes has been proposed for H. alvei, but it has not been correlated with known OPS structures, and no previous report has described the genetic features of OPS. In this study, we obtained the genome sequences of 21 H. alvei strains (as defined by previous immunochemical studies) with different lipopolysaccharides. This is the first study to show that the O-antigen gene cluster in H. alvei is located between mpo and gnd in the chromosome. All 21 of the OPS gene clusters contain both the wzx gene and the wzy gene and display a large number of polymorphisms. We developed an O serotype-specific wzy-based suspension array to detect all 21 of the distinct OPS forms we identified in H. alvei. To the best of our knowledge, this is the first report to identify the genetic features of H. alvei antigenic variation and to develop a molecular technique to identify and classify different serotypes.

structure, O-polysaccharide, O-antigens, Hafnia alvei, serotyping, genome, genomics, genetic diversity, O-antigen gene cluster, gene clusters, wzx, wzy, wzy gene

Structure type: polymer chemical repeating unit
Location inside paper: fig.3, H. alvei PCM 1223
Trivial name: mannose homopolysaccharide
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141111,IEDB_141795,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153756,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_164480,IEDB_174840,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72
 
Methods: PCR, genetic methods, bioinformatic analysis, function analysis of gene clusters
Biosynthesis and genetic data: the first attempt to locate the OPS gene cluster in the H. alvei genome
Comments, role: chemical repeat frame position was shifted
 
NCBI Taxonomy refs (TaxIDs): 569
Reference(s) to other database(s): GTC:G87424QV, GlycomeDB:668, CCSD:47538
Show glycosyltransferases
 

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