Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 27357519Publication DOI: 10.2217/fmb-2015-0028Journal NLM ID: 101278120Publisher: London: Future Medicine
Correspondence: jtomas
ub.edu
Institutions: Departamento de Microbiologia, Facultad de Biologia, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain, Institute of Virology, Hannover Medical School, Hannover, Germany
In the Wzx/Wzy-dependent assembled pathway, the assembled O-antigen repeat units are translocated from the cytosolic to the periplasmic face of the inner membrane by a Wzx translocase and then, polymerized by the integral membrane protein Wzy to form a glycan chain. We demonstrate that the activity of the Escherichia coli O-antigen polymerase (Wzy) is dependent on the first sugar of the O-antigen repeat unit to produce the O-antigen polymerization and therefore, there is a need for a concerted action with the enzyme transferring the initial HexNAc to undecaprenyl phosphate (UDP-HexNAc: polyprenol-P HexNAc-1-P transferase). Furthermore, in the case of Aeromonas hydrophila Wzy-O34 polymerization activity, the enzyme is permissive with the sugar at the nonreducing end of the O-antigen repeat unit.
Lipopolysaccharide, Escherichia coli, Aeromonas hydrophila, O-antigen polymerization, Wzy O-antigen assembly pathway
Structure type: polymer chemical repeating unit
Location inside paper: p.907, fig.1E, ECA
Trivial name: ECA, ECA LPS, enterobacterial common antigen (ECA)
Compound class: enterobacterial common antigen
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: PCR, GC-MS, SDS-PAGE, DNA techniques, acid hydrolysis, GLC, genetic methods, computer sequence analysis
Biosynthesis and genetic data: topology of O-antigen polymerases (Wzy)
Related record ID(s): 11356, 11786, 11787, 11788
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G63996VH, GlycomeDB:
7812
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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