Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
];
infection due to Shigella dysenteriae [ICD11:
XN285 ]
NCBI PubMed ID: 7392309Publication DOI: 10.1016/j.carres.2016.06.004Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: goran.widmalm
su.se
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh, Karolinska Institute, Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden
The structure of a polysaccharide from Vibrio parahaemolyticus strain AN-16000 has been investigated. The sugar and absolute configuration analysis revealed d-Glc, d-GalN, d-QuiN and l-FucN as major components. The PS was subjected to dephosphorylation with aqueous 40% HF to obtain an oligosaccharide that was analyzed by (1)H and (13)C NMR spectroscopy. The HR-MS spectrum of the oligosaccharide revealed a pentasaccharide composed of two Glc residues, one QuiNAc and one GalNAc, one FucNAc, as well as a glycerol moiety. The structure of the PS was determined using (1)H, (13)C, (15)N and (31)P NMR spectroscopy; inter-residue correlations were identified by (1)H,(13)C-heteronuclear multiple-bond correlation, (1)H,(1)H-NOESY and (1)H,(31)P-hetero-TOCSY experiments. The PS backbone has the following teichoic acid-like structure: →3)-d-Gro-(1-P-6)-β-d-Glcp-(1→4)-α-l-FucpNAc-(1→3)-β-d-QuipNAc-(1 → with a side-chain consisting of α-d-Glcp-(1→6)-α-d-GalpNAc-(1→ linked to the O3 position of the FucNAc residue.
NMR, Vibrio parahaemolyticus, dephosphorylation, Glycerol-1-phosphate
Structure type: suggested polymer biological repeating unit
Location inside paper: p.47, fig.7, E. coli O29, S. dysenteriae type 11
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130695,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_241118,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, HF solvolysis, sugar analysis, ESI-MS, acid hydrolysis, GLC, NMR-1D, GPC, mild acid degradation
Related record ID(s): 297, 299, 11374, 11595, 11800, 11801, 11803, 11804, 11805, 20208, 23077, 25744, 30874
NCBI Taxonomy refs (TaxIDs): 1095711,
622Reference(s) to other database(s): GTC:G55969UH
Show glycosyltransferases
There is only one chemically distinct structure:
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