Taxonomic group: animal / Cnidaria
(Phylum: Cnidaria)
Organ / tissue: perirhopalial tissue
Publication DOI: 10.1007/BF00318487Journal NLM ID: 0417625Publisher: Springer
Institutions: Whitney Laboratory, St. Augustine, FL, USA, Department of Neuroscience, University of Florida, Gainesville, FL, USA, Department of Physiology, University of Florida, Gainesville, FL, USA
Integral and membrane-associated proteins extracted from neuron-enriched perirhopalial tissue of the jellyfish Cyanea capillata were probed with a panel of lectins that recognize sugar epitopes of varying complexity. Of the 13 lectins tested, only concanavalin A, jacalin lectin and tomato lectin stained distinct bands on Western blots, indicating the presence of repeating α-1,6-mannoses, terminal Gal-α-1,6-GalNAc and repeating β-1,4-linked GlcNAc, respectively. In whole-mounted perirhopalial tissue, jacalin lectin stained several cell types, including neurons, muscle, cilia and mucus strands. Tomato lectin stained secretory cells intensely, and neurons in a punctate fashion. Concanavalin A stained cytoplasmic epitopes in both ecto-and endodermal cells, and ectodermal secretory cells and the mucus strands emanating from them. With the exception of tomato lectin's sugar epitope, the other sugar epitopes identified in this study are “non-complex.” This study suggests that while glycosylation of integral and membrane-associated proteins occurs in Cyanea, the sugars post-translationally linked to these proteins tend to be simple.
carbohydrate, linkage, sugar, Scyphozoa, nerve, Cyanea capillata (Cnidaria)
Structure type: homopolymer
Trivial name: LAM, mannan, galactomannan, cell wall heteromannan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, N-glycan, cell surface polysaccharide, mannan, D-mannan, galactomannan
Contained glycoepitopes: IEDB_130701,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Comments, role: fragment; sugar epitope recognized by concavaline A lectin from Jasminum officinale
NCBI Taxonomy refs (TaxIDs): 27804Reference(s) to other database(s): GTC:G55317BB, GlycomeDB:
6776, CCSD:
46066, CBank-STR:6345
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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