A water-insoluble, extracellular polysaccharide was isolated from the culture medium of the snow mold fungus, Microdochium nivale, that had been cultivated in potato/dextrose broth. The polysaccharide consisted of glucose only. Its Fourier transform infrared spectrum showed a beta configuration of the C1 position of glucose. Linkage analysis of the polysaccharide showed that it had a linear structure of β-(1→4)-linked glucose. The polysaccharide was therefore identified as cellulose. This is the first report of extracellular cellulose occurring in fungi.
extracellular polysaccharide, cellulose, β-glucan, Microdochium nivale, snow mold
Publication DOI: 10.1023/A:1005647928900Journal NLM ID: 8008051Publisher: Kluwer Academic Publishers
Correspondence: hoku@ees.hokudai.ac.jp
Institutions: Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, AIST/MITI, Toyohira-ku, Sapporo, 062-8517, Japan, Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan, National Institute of Agro-Environmental Sciences, Kan-non dai, Tsukuba, 305-8604, Japan, Hokkaido National Agricultural Experiment Station, Toyohira-ku, Sapporo, 062-8555, Japan
Methods: GC-MS, acid hydrolysis, GC, FTIR, enzymatic digestion, methylation analysis, gel permeation chromatography