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Found 3 records.
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1. (CSDB ID: 11915) | report error |
| Gly-(2-?)-a-D-GlcpN-(1-4)-a-D-GalpA-(1-3)-+ b-D-Glcp-(1-4)-+ | | a-D-GlcpN-(1-4)-a-D-GalpA-(1-7)-L-gro-a-D-manHepp-(1-7)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdo | b-D-Glcp-(1-2)-+ | Show graphically |
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Edwardsiella tarda EIB202
(NCBI TaxID 498217,
species name lookup)
]; meningitis [ICD11: 1D01 ]; osteomyelitis [ICD11: FB84 ]; cholecystitis [ICD11: DC12 ]
iitd.pan.wroc.pl>The chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry.
NMR, core oligosaccharide, MALDI-TOF MS, Edwardsiella tarda, ESI MSn, genomic
Structure type: oligomer ; 933.7 [M+Gly-2H]2-13C NMR data: Linkage Residue C1 C2 C3 C4 C5 C6 C7 C8 5,3,2 bDGlcp 103.2 74.5 76.3 71.6 76.5 62.7 5,3,3,4,? xXGly 168.0 41.8 5,3,3,4 aDGlcpN 97.0 55.1 70.5 70.5 72.8 62.5 5,3,3 aDGalpA 102.5 69.6 72.2 80.9 72.5 175.6 5,3,7,7,4 aDGlcpN 95.6 55.1 70.4 76.6 73.2 60.0 5,3,7,7 aDGalpA 99.8 69.0 69.9 77.9 70.9 176.7 5,3,7 aXLDmanHepp 103.2 71.1 72.3 71.4 73.4 69.1 72.0 5,3 aXLDmanHepp 99.6 78.6 80.0 66.6 73.4 69.3 73.3 5,4 bDGlcp 103.3 73.9 76.2 69.9 76.2 61.5 5 aXLDmanHepp 101.7 71.3 74.7 74.4 72.2 69.4 63.9 aXKdo? ? 97.7 34.7 66.5 75.7 70.1 66.9 64.3 1H NMR data: Linkage Residue H1 H2 H3 H4 H5 H6 H7 H8 5,3,2 bDGlcp 4.63 3.40 3.56 3.33 3.60 3.69-3.98 5,3,3,4,? xXGly - 3.90-4.09 5,3,3,4 aDGlcpN 5.29 3.39 3.99 3.63 4.27 3.69-3.97 5,3,3 aDGalpA 5.47 3.93 4.26 4.48 4.67 - 5,3,7,7,4 aDGlcpN 5.33 3.34 4.02 3.60 4.19 3.90-3.91 5,3,7,7 aDGalpA 5.12 4.05 4.16 4.62 4.41 - 5,3,7 aXLDmanHepp 4.98 4.05 3.95 3.93 3.72 4.23 3.69-4.88 5,3 aXLDmanHepp 5.44 4.34 4.19 4.12 3.67 4.28 3.62-4.01 5,4 bDGlcp 4.51 3.36 3.55 3.56 3.55 3.87-3.95 5 aXLDmanHepp 5.19 4.15 4.25 4.35 4.26 4.18 3.80 aXKdo? - - 1.96-2.29 4.21 4.25 3.81 3.97 3.69-3.92 1H/13C HSQC data: Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7 C8/H8 5,3,2 bDGlcp 103.2/4.63 74.5/3.40 76.3/3.56 71.6/3.33 76.5/3.60 62.7/3.69-3.98 5,3,3,4,? xXGly 41.8/3.90-4.09 5,3,3,4 aDGlcpN 97.0/5.29 55.1/3.39 70.5/3.99 70.5/3.63 72.8/4.27 62.5/3.69-3.97 5,3,3 aDGalpA 102.5/5.47 69.6/3.93 72.2/4.26 80.9/4.48 72.5/4.67 5,3,7,7,4 aDGlcpN 95.6/5.33 55.1/3.34 70.4/4.02 76.6/3.60 73.2/4.19 60.0/3.90-3.91 5,3,7,7 aDGalpA 99.8/5.12 69.0/4.05 69.9/4.16 77.9/4.62 70.9/4.41 5,3,7 aXLDmanHepp 103.2/4.98 71.1/4.05 72.3/3.95 71.4/3.93 73.4/3.72 69.1/4.23 72.0/3.69-4.88 5,3 aXLDmanHepp 99.6/5.44 78.6/4.34 80.0/4.19 66.6/4.12 73.4/3.67 69.3/4.28 73.3/3.62-4.01 5,4 bDGlcp 103.3/4.51 73.9/3.36 76.2/3.55 69.9/3.56 76.2/3.55 61.5/3.87-3.95 5 aXLDmanHepp 101.7/5.19 71.3/4.15 74.7/4.25 74.4/4.35 72.2/4.26 69.4/4.18 63.9/3.80 aXKdo? 34.7/1.96-2.29 66.5/4.21 75.7/4.25 70.1/3.81 66.9/3.97 64.3/3.69-3.92
1H NMR data:
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13C NMR data:
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The spectrum also has 1 signal at unknown position (not plotted). |
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2. (CSDB ID: 12108) | report error |
| a-D-GlcpN-(1-4)-a-D-GalpA-(1-7)-L-gro-a-D-manHepp-(1-7)-+ | Gly-(2-?)-+ | b-D-Glcp-(1-4)-+ | | | b-D-Galp-(1-3)-a-D-GlcpNAc-(1-4)-a-D-GalpA-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdo | b-D-Glcp-(1-2)-+ | Show graphically |
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Show legend Show as text |
Edwardsiella tarda EIB202
(NCBI TaxID 498217,
species name lookup)
]; meningitis [ICD11: 1D01 ]; osteomyelitis [ICD11: FB84 ]; cholecystitis [ICD11: DC12 ]iitd.pan.wroc.pl>The chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry.
NMR, core oligosaccharide, MALDI-TOF MS, Edwardsiella tarda, ESI MSn, genomic
Structure type: oligomer13C NMR data: Linkage Residue C1 C2 C3 C4 C5 C6 C7 C8 5,3,2 bDGlcp 103.2 74.5 76.3 71.6 76.5 62.7 5,3,3,4,3 bDGalp 103.6 71.0 72.7 71.4 75.1 63.2 5,3,3,4,2 Ac 175.9 23.2 5,3,3,4,? xXGly 168.0 41.8 5,3,3,4 aDGlcpN 99.8 54.6 71.6 70.2 73.2 61.0 5,3,3 aDGalpA 102.5 69.6 72.2 80.9 72.5 175.6 5,3,7,7,4 aDGlcpN 95.6 55.1 70.4 76.6 73.2 60.0 5,3,7,7 aDGalpA 99.8 69.0 69.9 77.9 70.9 176.7 5,3,7 aXLDmanHepp 103.2 71.1 72.3 71.4 73.4 69.1 72.0 5,3 aXLDmanHepp 99.6 78.6 80.0 66.6 73.4 69.3 73.3 5,4 bDGlcp 103.3 73.9 76.2 69.9 76.2 61.5 5 aXLDmanHepp 101.7 71.3 74.7 74.4 72.2 69.4 63.9 aXKdo? ? 97.7 34.7 66.5 75.7 70.1 66.9 64.3 1H NMR data: Linkage Residue H1 H2 H3 H4 H5 H6 H7 H8 5,3,2 bDGlcp 4.63 3.40 3.56 3.33 3.60 3.69-3.98 5,3,3,4,3 bDGalp 4.45 3.61 3.73 3.93 3.59 3.74-3.80 5,3,3,4,2 Ac - 2.13 5,3,3,4,? xXGly - 3.90-4.09 5,3,3,4 aDGlcpN 5.06 4.02 3.83 3.63 4.28 3.90-3.91 5,3,3 aDGalpA 5.47 3.93 4.26 4.48 4.67 - 5,3,7,7,4 aDGlcpN 5.33 3.34 4.02 3.60 4.19 3.90-3.91 5,3,7,7 aDGalpA 5.12 4.05 4.16 4.62 4.41 - 5,3,7 aXLDmanHepp 4.98 4.05 3.95 3.93 3.72 4.23 3.69-4.88 5,3 aXLDmanHepp 5.44 4.34 4.19 4.12 3.67 4.28 3.62-4.01 5,4 bDGlcp 4.51 3.36 3.55 3.56 3.55 3.87-3.95 5 aXLDmanHepp 5.19 4.15 4.25 4.35 4.26 4.18 3.80 aXKdo? - - 1.96-2.29 4.21 4.25 3.81 3.97 3.69-3.92 1H/13C HSQC data: Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7 C8/H8 5,3,2 bDGlcp 103.2/4.63 74.5/3.40 76.3/3.56 71.6/3.33 76.5/3.60 62.7/3.69-3.98 5,3,3,4,3 bDGalp 103.6/4.45 71.0/3.61 72.7/3.73 71.4/3.93 75.1/3.59 63.2/3.74-3.80 5,3,3,4,2 Ac 23.2/2.13 5,3,3,4,? xXGly 41.8/3.90-4.09 5,3,3,4 aDGlcpN 99.8/5.06 54.6/4.02 71.6/3.83 70.2/3.63 73.2/4.28 61.0/3.90-3.91 5,3,3 aDGalpA 102.5/5.47 69.6/3.93 72.2/4.26 80.9/4.48 72.5/4.67 5,3,7,7,4 aDGlcpN 95.6/5.33 55.1/3.34 70.4/4.02 76.6/3.60 73.2/4.19 60.0/3.90-3.91 5,3,7,7 aDGalpA 99.8/5.12 69.0/4.05 69.9/4.16 77.9/4.62 70.9/4.41 5,3,7 aXLDmanHepp 103.2/4.98 71.1/4.05 72.3/3.95 71.4/3.93 73.4/3.72 69.1/4.23 72.0/3.69-4.88 5,3 aXLDmanHepp 99.6/5.44 78.6/4.34 80.0/4.19 66.6/4.12 73.4/3.67 69.3/4.28 73.3/3.62-4.01 5,4 bDGlcp 103.3/4.51 73.9/3.36 76.2/3.55 69.9/3.56 76.2/3.55 61.5/3.87-3.95 5 aXLDmanHepp 101.7/5.19 71.3/4.15 74.7/4.25 74.4/4.35 72.2/4.26 69.4/4.18 63.9/3.80 aXKdo? 34.7/1.96-2.29 66.5/4.21 75.7/4.25 70.1/3.81 66.9/3.97 64.3/3.69-3.92
1H NMR data:
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13C NMR data:
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The spectrum also has 1 signal at unknown position (not plotted). |
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3. (CSDB ID: 25510) | report error |
| L-Thr-(2-6)-a-D-GalpA-(1-3)-+ | -3)-b-D-GlcpNAc-(1-4)-b-D-GalpNAc-(1-3)-a-D-Galp-(1-4)-a-L-Rhap-(1- | Show graphically |
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Edwardsiella tarda MT 108
(Ancestor NCBI TaxID 636,
species name lookup)
]; infectious gastroenteritis [ICD11: 1A40.Z ]; gas gangrene [ICD11: 1C16 , ICD11: XN7J5 ]; meningitis [ICD11: 1D01 ]; osteomyelitis [ICD11: FB84 ]; cholecystitis [ICD11: DC12 ]dfo-mpo.gc.caMass spectrometric studies are now playing a leading role in the elucidation of lipopolysaccharide (LPS) structures through the characterization of antigenic polysaccharides, core oligosaccharides and lipid A components including LPS genetic modifications. The conventional MS and MS/MS analyses together with CID fragmentation provide additional structural information complementary to the previous analytical experiments, and thus contribute to an integrated strategy for the simultaneous characterization and correct sequencing of the carbohydrate moiety.
LPS, O-antigen, lipid A, core oligosaccharide, MS and MS/MS analyses
Structure type: polymer chemical repeating unit| New query | Export IDs | Home | Help |
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