Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: gastroenteritis [ICD11:
1A40.0 
];
diarrhea [ICD11:
ME05.1 , ICD11:
SA55 ]
The structure was elucidated in this paperNCBI PubMed ID: 28088128Publication DOI: 10.1016/j.carres.2016.12.006Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: aturska
hektor.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland, Department of Fish Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland
The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas hydrophila strain K691 and studied by chemical methods and 1H and 13C NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, NOESY, 1H-detected heteronuclear 1H,13C HSQC, and HMBC experiments. It was found that the O-specific polysaccharide was built up of pentasaccharide repeating units composed of β-GlcpNAc, 2-O-acetylated α-Rhap, and β-Quip4NAc residues. The following structure of the OPS was established: →3)-α-l-Rha2OAc-(1→3)-β-d-GlcNAc-(1→3)-α-l-Rha2OAc-(1→3)-β- d-GlcNAc-(1→2)-β-d-Qui4NAc-(1→.
LPS, O-antigen, O-specific polysaccharide, O-acetylation, 6-dideoxy-d-glucose, 4-amino-4, Aeromonas hydrophila, Fish pathogen
Structure type: polymer chemical repeating unit
Location inside paper: abtract, table 1, p.26
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130422,IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, sugar analysis, acid hydrolysis, GPC, reduction with NaBD4, N-acetylation
NCBI Taxonomy refs (TaxIDs): 644Reference(s) to other database(s): GTC:G26193UK
Show glycosyltransferases
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,3,3,3,2 Ac 174.3 21.6
2,3,3,3 aLRhap 99.7 72.6 80.8 72.1 69.5 17.7
2,3,3,2 Ac 176.0 23.6-23.7
2,3,3 bDGlcpN 102.8 56.8 83.5 69.0 77.0 61.6
2,3,2 Ac 174.3 21.6
2,3 aLRhap 99.6 72.8 80.0 72.0 70.0 17.6
2,2 Ac 176.0 23.6-23.7
2 bDGlcpN 102.7 56.8 83.4 69.7 77.3 61.8
4 Ac 176.0 23.6-23.7
bDQuip4N 102.8 82.3 75.2 58.0 72.0 18.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,3,3,3,2 Ac - 2.16-2.17
2,3,3,3 aLRhap 4.92 5.08 4.05 3.70 4.14 1.30
2,3,3,2 Ac - 2.06-2.10
2,3,3 bDGlcpN 4.67 3.83 3.59 3.58 3.43 3.83-3.97
2,3,2 Ac - 2.16-2.17
2,3 aLRhap 4.89 5.11 3.94 3.55 4.06 1.26
2,2 Ac - 2.06-2.10
2 bDGlcpN 4.84 3.82 3.59 3.51 3.51 3.77-3.98
4 Ac - 2.06-2.10
bDQuip4N 4.64 3.54 3.55 3.54 3.53 1.14
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,3,3,3,2 Ac 21.6/2.16-2.17
2,3,3,3 aLRhap 99.7/4.92 72.6/5.08 80.8/4.05 72.1/3.70 69.5/4.14 17.7/1.30
2,3,3,2 Ac 23.6-23.7/2.06-2.10
2,3,3 bDGlcpN 102.8/4.67 56.8/3.83 83.5/3.59 69.0/3.58 77.0/3.43 61.6/3.83-3.97
2,3,2 Ac 21.6/2.16-2.17
2,3 aLRhap 99.6/4.89 72.8/5.11 80.0/3.94 72.0/3.55 70.0/4.06 17.6/1.26
2,2 Ac 23.6-23.7/2.06-2.10
2 bDGlcpN 102.7/4.84 56.8/3.82 83.4/3.59 69.7/3.51 77.3/3.51 61.8/3.77-3.98
4 Ac 23.6-23.7/2.06-2.10
bDQuip4N 102.8/4.64 82.3/3.54 75.2/3.55 58.0/3.54 72.0/3.53 18.0/1.14
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,3,3,3,2 | Ac |
| 2.16
2.17 | |
| 2,3,3,3 | aLRhap | 4.92 | 5.08 | 4.05 | 3.70 | 4.14 | 1.30 |
| 2,3,3,2 | Ac |
| 2.06
2.10 | |
| 2,3,3 | bDGlcpN | 4.67 | 3.83 | 3.59 | 3.58 | 3.43 | 3.83
3.97 |
| 2,3,2 | Ac |
| 2.16
2.17 | |
| 2,3 | aLRhap | 4.89 | 5.11 | 3.94 | 3.55 | 4.06 | 1.26 |
| 2,2 | Ac |
| 2.06
2.10 | |
| 2 | bDGlcpN | 4.84 | 3.82 | 3.59 | 3.51 | 3.51 | 3.77
3.98 |
| 4 | Ac |
| 2.06
2.10 | |
| | bDQuip4N | 4.64 | 3.54 | 3.55 | 3.54 | 3.53 | 1.14 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,3,3,3,2 | Ac | 174.3 | 21.6 | |
| 2,3,3,3 | aLRhap | 99.7 | 72.6 | 80.8 | 72.1 | 69.5 | 17.7 |
| 2,3,3,2 | Ac | 176.0 | 23.6
23.7 | |
| 2,3,3 | bDGlcpN | 102.8 | 56.8 | 83.5 | 69.0 | 77.0 | 61.6 |
| 2,3,2 | Ac | 174.3 | 21.6 | |
| 2,3 | aLRhap | 99.6 | 72.8 | 80.0 | 72.0 | 70.0 | 17.6 |
| 2,2 | Ac | 176.0 | 23.6
23.7 | |
| 2 | bDGlcpN | 102.7 | 56.8 | 83.4 | 69.7 | 77.3 | 61.8 |
| 4 | Ac | 176.0 | 23.6
23.7 | |
| | bDQuip4N | 102.8 | 82.3 | 75.2 | 58.0 | 72.0 | 18.0 |
|
There is only one chemically distinct structure:
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