Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Enterobacter cloacae [ICD11:
XN3YM ]
The structure was elucidated in this paperNCBI PubMed ID: 28645003Publication DOI: 10.1016/j.carres.2017.05.021Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel
ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, 300071 Tianjin, China
An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C4115 and studied by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit of the O-polysaccharide was established: →2)-α-l-Rhap-(1→2)-α-l-Rhap-(1→2)-α-l-Rhap-(1→2)-α-d-Galp-(1→3)-α-d-FucpNAc-(1→. The O-antigen gene cluster of E. cloacae C4115 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in consistence with the O-polysaccharide structure. The O-antigen structure and gene cluster of E. cloacae C4115 are similar to those of E. cloacae G3421 studied by us earlier (Perepelov A.V. et al. Carbohydr. Res. 427 (2016) 55-59).
Lipopolysaccharide, O-antigen, bacterial polysaccharide structure, O-antigen gene cluster
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1, p.112, chart 1 E. cloacae C4115
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130669,IEDB_133754,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_144825,IEDB_151528,IEDB_190606,IEDB_225177,IEDB_885823,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, DNA sequencing, SDS-PAGE, sugar analysis, acid hydrolysis, GLC, GPC, delipidation, function analysis of gene clusters
Related record ID(s): 12196
NCBI Taxonomy refs (TaxIDs): 550Reference(s) to other database(s): GTC:G51475VE
Show glycosyltransferases
NMR conditions: in D2O at 323 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2,2,2 aLRhap 100.6 77.3 70.6 73.4 70.7 18.1
3,2,2 aLRhap 101.9 79.7 71.3 73.5 70.6 17.9
3,2 aLRhap 101.3 78.9 71.2 73.3 70.7 18.0
3 aDGalp 96.3 74.4 70.8 71.0 72.6 62.3
2 Ac 175.5 23.5
aDFucpN 97.5 48.9 74.4 69.2 68.2 16.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2,2,2 aLRhap 4.99 4.04 3.90 3.47 3.76 1.29
3,2,2 aLRhap 5.12 4.07 3.87 3.45 3.70 1.27
3,2 aLRhap 5.23 4.06 3.88 3.50 3.69 1.32
3 aDGalp 5.14 3.96 3.96 3.98 3.93 3.75-3.78
2 Ac - 2.06
aDFucpN 4.97 4.36 4.06 3.99 4.35 1.24
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2,2,2 aLRhap 100.6/4.99 77.3/4.04 70.6/3.90 73.4/3.47 70.7/3.76 18.1/1.29
3,2,2 aLRhap 101.9/5.12 79.7/4.07 71.3/3.87 73.5/3.45 70.6/3.70 17.9/1.27
3,2 aLRhap 101.3/5.23 78.9/4.06 71.2/3.88 73.3/3.50 70.7/3.69 18.0/1.32
3 aDGalp 96.3/5.14 74.4/3.96 70.8/3.96 71.0/3.98 72.6/3.93 62.3/3.75-3.78
2 Ac 23.5/2.06
aDFucpN 97.5/4.97 48.9/4.36 74.4/4.06 69.2/3.99 68.2/4.35 16.7/1.24
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2,2,2 | aLRhap | 4.99 | 4.04 | 3.90 | 3.47 | 3.76 | 1.29 |
| 3,2,2 | aLRhap | 5.12 | 4.07 | 3.87 | 3.45 | 3.70 | 1.27 |
| 3,2 | aLRhap | 5.23 | 4.06 | 3.88 | 3.50 | 3.69 | 1.32 |
| 3 | aDGalp | 5.14 | 3.96 | 3.96 | 3.98 | 3.93 | 3.75
3.78 |
| 2 | Ac |
| 2.06 | |
| | aDFucpN | 4.97 | 4.36 | 4.06 | 3.99 | 4.35 | 1.24 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2,2,2 | aLRhap | 100.6 | 77.3 | 70.6 | 73.4 | 70.7 | 18.1 |
| 3,2,2 | aLRhap | 101.9 | 79.7 | 71.3 | 73.5 | 70.6 | 17.9 |
| 3,2 | aLRhap | 101.3 | 78.9 | 71.2 | 73.3 | 70.7 | 18.0 |
| 3 | aDGalp | 96.3 | 74.4 | 70.8 | 71.0 | 72.6 | 62.3 |
| 2 | Ac | 175.5 | 23.5 | |
| | aDFucpN | 97.5 | 48.9 | 74.4 | 69.2 | 68.2 | 16.7 |
|
There is only one chemically distinct structure:
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Found 1 record.
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