Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Organ / tissue: gastrointestinal tract,
upper airwaysAssociated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
The structure was elucidated in this paperNCBI PubMed ID: 26723873Publication DOI: 10.1016/j.ijmm.2015.12.002Journal NLM ID: 100898849Publisher: Jena, Germany: Urban & Fischer Verlag
Correspondence: Gabor Nagy <gabor.nagy
arsanis.com>
Institutions: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Arsanis Biosciences GmbH, Vienna, Austria
Klebsiella pneumoniae ST258 is a globally disseminated, extremely drug resistant, nosocomial clone with limited treatment options. We show that the vast majority of ST258 isolates express modified d-galactan-I lipopolysaccharide O-antigen, termed hereinafter as D-galactan-III. The genetic determinant required for galactan-III synthesis was identified as a distinct operon adjacent to the rfb (wb) locus encoding D-galactan-I synthesis. The three genes within the operon encode predicted glycosyltransferases. Testing an isogenic transformant pair revealed that expression of D-galactan-III, in comparison to D-galactan-I, conferred improved survival in the presence of human serum. Eighty-three percent of the more than 200 ST258 draft genome sequences currently available carries the corresponding operon and hence these isolates are predicted to express galactan-III antigens. A D-galactan-III specific monoclonal antibody (mAb) was shown to bind to extracted LPS from a panel of ST258 isolates. The same mAb confirmed accessibility of galactan-III in surface staining of ST258 irrespective of the distinct capsular antigens expressed by both clades described previously. Based on these data, the galactan-III antigen may represent an attractive target for active and passive immunization approaches against K. pneumoniae ST258.
monoclonal antibody, LPS O-antigen, d-galactan-I, d-galactan-III, Klebsiella pneumoniae ST258, Serotype O2
Structure type: polymer chemical repeating unit
Location inside paper: table 2, PCM-27, p.94, fig.4, PCM-27, Kp26/pKP100, D-galactan-III
Trivial name: D-galactan-III, D-galactan III
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_144987,IEDB_151528,IEDB_190606,SB_31,SB_7
Methods: 13C NMR, 1H NMR, methylation, gel filtration, NMR-2D, sugar analysis, ELISA, mild acid hydrolysis, genetic methods, immunoblotting, serum bactericidal assays, HR-MAS NMR, statistical analysis, cloning, bioinformatic analysis, immunization, flow cytometry analysis
Related record ID(s): 12240
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G38255JW, GlycomeDB:
16920
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3 bDGalf 110.5 81.4 85.6 80.5 71.0 63.6
4 aDGalp 101.2 69.8 70.0 69.5 71.5 60.8
aDGalp 101.0 68.6 77.7 79.1 73.1 61.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3 bDGalf 5.22 4.34 4.08 4.30 3.86 3.72
4 aDGalp 5.01 3.83 3.92 4.07 4.23 3.79-3.83
aDGalp 5.10 4.10 3.95 4.18 4.18 3.84-3.91
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3 bDGalf 110.5/5.22 81.4/4.34 85.6/4.08 80.5/4.30 71.0/3.86 63.6/3.72
4 aDGalp 101.2/5.01 69.8/3.83 70.0/3.92 69.5/4.07 71.5/4.23 60.8/3.79-3.83
aDGalp 101.0/5.10 68.6/4.10 77.7/3.95 79.1/4.18 73.1/4.18 61.1/3.84-3.91
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3 | bDGalf | 5.22 | 4.34 | 4.08 | 4.30 | 3.86 | 3.72 |
| 4 | aDGalp | 5.01 | 3.83 | 3.92 | 4.07 | 4.23 | 3.79
3.83 |
| | aDGalp | 5.10 | 4.10 | 3.95 | 4.18 | 4.18 | 3.84
3.91 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3 | bDGalf | 110.5 | 81.4 | 85.6 | 80.5 | 71.0 | 63.6 |
| 4 | aDGalp | 101.2 | 69.8 | 70.0 | 69.5 | 71.5 | 60.8 |
| | aDGalp | 101.0 | 68.6 | 77.7 | 79.1 | 73.1 | 61.1 |
|
There is only one chemically distinct structure:
Found 
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