Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: dysentery [ICD11:
SA56 
];
diarrhea [ICD11:
ME05.1 , ICD11:
SA55 ];
gastroenteritis [ICD11:
1A40.0 ]
The structure was elucidated in this paperNCBI PubMed ID: 29186063Publication DOI: 10.3390/ijms18122572Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: tomasz.niedziela
iitd.pan.wroc.pl
Institutions: Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland
The high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR) analysis of Plesiomonas shigelloides 78/89 lipopolysaccharide directly on bacteria revealed the characteristic structural features of the O-acetylated polysaccharide in the NMR spectra. The O-antigen profiles were unique, yet the pattern of signals in the, spectra along with their (1)H,13C chemical shift values, resembled these of d-galactan I of Klebsiella pneumoniae. The isolated O-specific polysaccharide (O-PS) of P. shigelloides strain CNCTC 78/89 was investigated by 1H and 13C NMR spectroscopy, mass spectrometry and chemical methods. The analyses demonstrated that the P. shigelloides 78/89 O-PS is composed of →3)-α-d-Galp-(1→3)-β-d-Galf2OAc-(1→ disaccharide repeating units. The O-acetylation was incomplete and resulted in a microheterogeneity of the O-antigen. This O-acetylation generates additional antigenic determinants within the O-antigen, forms a new chemotype, and contributes to the epitopes recognized by the O-serotype specific antibodies. The serological cross-reactivities further confirmed the inter-specific structural similarity of these O-antigens.
Lipopolysaccharide, O-antigen, NMR spectroscopy, O-acetylation, HR-MAS, Plesiomonas shigelloides, d-galactan I
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1
Trivial name: D-galactan I
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
Methods: 13C NMR, 1H NMR, gel filtration, SDS-PAGE, sugar analysis, ELISA, mild acid hydrolysis, MALDI-TOF MS, immunoblotting, HR-MAS NMR, acetylation
Comments, role: O-acetylated glycoform of OPS
Related record ID(s): 12335
NCBI Taxonomy refs (TaxIDs): 703Reference(s) to other database(s): GTC:G87851RZ
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2 32%Ac 173.1 20.5
3 bDGalf 107.1 81.8 82.1 82.9 70.8 62.7
aDGalp 99.4 67.2 76.7 69.1 71.3 60.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2 32%Ac - 2.11
3 bDGalf 5.32 5.25 4.28 4.32 3.89 3.65-3.68
aDGalp 5.08 3.94 3.95 4.11 4.11 3.62-3.69
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2 32%Ac 20.5/2.11
3 bDGalf 107.1/5.32 81.8/5.25 82.1/4.28 82.9/4.32 70.8/3.89 62.7/3.65-3.68
aDGalp 99.4/5.08 67.2/3.94 76.7/3.95 69.1/4.11 71.3/4.11 60.9/3.62-3.69
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2 | 32%Ac |
| 2.11 | |
| 3 | bDGalf | 5.32 | 5.25 | 4.28 | 4.32 | 3.89 | 3.65
3.68 |
| | aDGalp | 5.08 | 3.94 | 3.95 | 4.11 | 4.11 | 3.62
3.69 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2 | 32%Ac | 173.1 | 20.5 | |
| 3 | bDGalf | 107.1 | 81.8 | 82.1 | 82.9 | 70.8 | 62.7 |
| | aDGalp | 99.4 | 67.2 | 76.7 | 69.1 | 71.3 | 60.9 |
|
There is only one chemically distinct structure:
Found 
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