Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Homo sapiens
Associated disease: septicemia [ICD11:
MA15.Y 
];
meningitis [ICD11:
1D01 ];
infection due to Streptococcus pneumoniae [ICD11:
XN3PW ]
NCBI PubMed ID: 28973947Publication DOI: 10.1073/pnas.1706875114Journal NLM ID: 7505876Publisher: National Academy of Sciences
Correspondence: claney.pereira
vaxxilon.com; peter.seeberger
mpikg.mpg.de
Institutions: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, D-14424 Potsdam, Germany, Department of Chemistry and Biochemistry, Freie Universität Berlin, D-14195 Berlin, Germany, Department of Infectious Diseases and Pulmonary Medicine, Charité - Universitätsmedizin Berlin, 10117 Berlin, Germany
Glycoconjugate vaccines based on isolated capsular polysaccharide (CPS) save millions of lives annually by preventing invasive pneumococcal disease caused by Streptococcus pneumoniae Some components of the S. pneumoniae glycoconjugate vaccine Prevnar13 that contains CPS antigens from 13 serotypes undergo modifications or degradation during isolation and conjugation, resulting in production problems and lower efficacy. We illustrate how stable, synthetic oligosaccharide analogs of labile CPS induce a specific protective immune response against native CPS using S. pneumoniae serotype 5 (ST-5), a problematic CPS component of Prevnar13. The rare aminosugar l-PneuNAc and a branched l-FucNAc present in the natural repeating unit (RU) are essential for antibody recognition and avidity. The epitope responsible for specificity differs from the part of the antigen that is stabilized by chemical modification. Glycoconjugates containing stable, monovalent synthetic oligosaccharide analogs of ST-5 CPS RU induced long-term memory and protective immune responses in rabbits superior to those elicited by the ST-5 CPS component in multivalent Prevnar13.
vaccine, glycoconjugate, S. pneumoniae, carbohydrate chemistry, serotype 5
Structure type: polymer chemical repeating unit
Location inside paper: fig.1A, ST-5 RU
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_142488,IEDB_146664,IEDB_423153,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, IR, SDS-PAGE, TLC, ELISA, chemical synthesis, biological assays, glycosylation, optical rotation measurement, statistical analysis, immunization, conjugation, HR-ESI-MS, microarray analysis, flow cytometry, microarray binding assays
NCBI Taxonomy refs (TaxIDs): 1313
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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