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Bouchez V, Albitar-Nehme S, Novikov A, Guiso N, Caroff M
Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541
International Journal of Molecular Sciences 18(5) (2017)
E1080
|
2HOLau-(1-3)-3HOMyr-(1-2)-+
|
3HOMyr-(1-3)-+ |
| |
2HOMyr-(1-3)-3HOMyr-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
| |
P-4)-+ 3HODco-(1-3)-+ |
Show graphically |
Bordetella holmesii FR4020
(Ancestor NCBI TaxID 35814,
species name lookup)
Bordetella holmesii BH01
(Ancestor NCBI TaxID 35814,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Organ / tissue: nasopharyngeal swabs,
bloodAssociated disease: infection due to Bordetella [ICD11:
XN9W3 
];
sickle cell anemia [ICD11:
3A51 ]
The structure was elucidated in this paperNCBI PubMed ID: 28524084Publication DOI: 10.3390/ijms18051080Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: martine.caroff
u-psud.fr
Institutions: Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, 25 rue du Dr Roux, 75724 Paris, France, Institute for integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique (CEA), Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud, Université Paris-Saclay, Orsay, France, LPS-BioSciences, I2BC, Bâtiment 409, Université de Paris-Sud, Orsay, France
Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography-mass spectrometry (GC-MS), and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). The analyses revealed a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to B. avium, B. hinzii and B. trematum lipids A, the hydroxytetradecanoic acid at the C-2' position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the isolates. This trait was only described in B. pertussis and B. parapertussis strains, as well as in B. petrii isolates by the past. The genetic bases for most of the key structural elements of lipid A were analyzed and supported the structural data.
structure, lipid A, endotoxin, mass spectrometry, genomic, Bordetella holmesii, Bordetellae
Structure type: oligomer ; 1773.25 [M-H]-
Location inside paper: fig.5, lipid A BH FR4020, lipid A Bh01
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531
Methods: methylation, PCR, DNA sequencing, GC-MS, de-O-acylation, TLC, acid hydrolysis, MALDI-MS, composition analysis, extraction, bioinformatic analysis
Biosynthesis and genetic data: genetic data
Comments, role: proposed structure of the main lipid A from B. holmesii FR4020 and B. holmesii BH01
Related record ID(s): 12438, 12439, 12440, 12441, 12442, 12443
NCBI Taxonomy refs (TaxIDs): 35814
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There is only one chemically distinct structure:
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Bouchez V, Albitar-Nehme S, Novikov A, Guiso N, Caroff M
Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541
International Journal of Molecular Sciences 18(5) (2017)
E1080
|
2HOLau-(1-3)-3HOMyr-(1-2)-+
|
3HOMyr-(1-3)-+ |
| |
2HOMyr-(1-3)-3HOMyr-(1-2)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
|
P-4)-+ |
Show graphically |
Bordetella holmesii FR4020
(Ancestor NCBI TaxID 35814,
species name lookup)
Bordetella holmesii BH01
(Ancestor NCBI TaxID 35814,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Organ / tissue: nasopharyngeal swabs,
bloodAssociated disease: infection due to Bordetella [ICD11:
XN9W3 ];
sickle cell anemia [ICD11:
3A51 ]
The structure was elucidated in this paperNCBI PubMed ID: 28524084Publication DOI: 10.3390/ijms18051080Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: martine.caroff
u-psud.fr
Institutions: Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, 25 rue du Dr Roux, 75724 Paris, France, Institute for integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique (CEA), Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud, Université Paris-Saclay, Orsay, France, LPS-BioSciences, I2BC, Bâtiment 409, Université de Paris-Sud, Orsay, France
Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography-mass spectrometry (GC-MS), and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). The analyses revealed a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to B. avium, B. hinzii and B. trematum lipids A, the hydroxytetradecanoic acid at the C-2' position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the isolates. This trait was only described in B. pertussis and B. parapertussis strains, as well as in B. petrii isolates by the past. The genetic bases for most of the key structural elements of lipid A were analyzed and supported the structural data.
structure, lipid A, endotoxin, mass spectrometry, genomic, Bordetella holmesii, Bordetellae
Structure type: oligomer ; 1603.0 [M-H]-
Location inside paper: fig.5, lipid A BH FR4020, lipid A Bh01
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_141807,IEDB_151531
Methods: methylation, PCR, DNA sequencing, GC-MS, de-O-acylation, TLC, acid hydrolysis, MALDI-MS, composition analysis, extraction, bioinformatic analysis
Biosynthesis and genetic data: genetic data
Comments, role: proposed structure of the main lipid A from B. holmesii FR4020 and B. holmesii BH01
Related record ID(s): 12056, 12439, 12440, 12441, 12442, 12443
NCBI Taxonomy refs (TaxIDs): 35814
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There is only one chemically distinct structure:
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