Department of Biotechnology and Molecular Biology, University of Opole, PL-Opole, Poland, Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Department of Microbiology, University of Barcelona, Diagonal 643, 08071 Barcelona, Spain
The chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry.
13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, 31P NMR, ESI-MS, mild acid hydrolysis, MALDI-TOF MS, function analysis of gene clusters
the NMR solution was not indicated; The positions of glycine in OSVIII and OSVII were not determined.
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7 C8
5,3,2 bDGlcp 103.2 74.5 76.3 71.6 76.5 62.7
5,3,3,4,3 bDGalp 103.6 71.0 72.7 71.4 75.1 63.2
5,3,3,4,2 Ac 175.9 23.2
5,3,3,4,? xXGly 168.0 41.8
5,3,3,4 aDGlcpN 99.8 54.6 71.6 70.2 73.2 61.0
5,3,3 aDGalpA 102.5 69.6 72.2 80.9 72.5 175.6
5,3,7,7,4 aDGlcpN 95.6 55.1 70.4 76.6 73.2 60.0
5,3,7,7 aDGalpA 99.8 69.0 69.9 77.9 70.9 176.7
5,3,7 aXLDmanHepp 103.2 71.1 72.3 71.4 73.4 69.1 72.0
5,3 aXLDmanHepp 99.6 78.6 80.0 66.6 73.4 69.3 73.3
5,4 bDGlcp 103.3 73.9 76.2 69.9 76.2 61.5
5 aXLDmanHepp 101.7 71.3 74.7 74.4 72.2 69.4 63.9
aXKdo? ? 97.7 34.7 66.5 75.7 70.1 66.9 64.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7 H8
5,3,2 bDGlcp 4.63 3.40 3.56 3.33 3.60 3.69-3.98
5,3,3,4,3 bDGalp 4.45 3.61 3.73 3.93 3.59 3.74-3.80
5,3,3,4,2 Ac - 2.13
5,3,3,4,? xXGly - 3.90-4.09
5,3,3,4 aDGlcpN 5.06 4.02 3.83 3.63 4.28 3.90-3.91
5,3,3 aDGalpA 5.47 3.93 4.26 4.48 4.67 -
5,3,7,7,4 aDGlcpN 5.33 3.34 4.02 3.60 4.19 3.90-3.91
5,3,7,7 aDGalpA 5.12 4.05 4.16 4.62 4.41 -
5,3,7 aXLDmanHepp 4.98 4.05 3.95 3.93 3.72 4.23 3.69-4.88
5,3 aXLDmanHepp 5.44 4.34 4.19 4.12 3.67 4.28 3.62-4.01
5,4 bDGlcp 4.51 3.36 3.55 3.56 3.55 3.87-3.95
5 aXLDmanHepp 5.19 4.15 4.25 4.35 4.26 4.18 3.80
aXKdo? - - 1.96-2.29 4.21 4.25 3.81 3.97 3.69-3.92
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7 C8/H8
5,3,2 bDGlcp 103.2/4.63 74.5/3.40 76.3/3.56 71.6/3.33 76.5/3.60 62.7/3.69-3.98
5,3,3,4,3 bDGalp 103.6/4.45 71.0/3.61 72.7/3.73 71.4/3.93 75.1/3.59 63.2/3.74-3.80
5,3,3,4,2 Ac 23.2/2.13
5,3,3,4,? xXGly 41.8/3.90-4.09
5,3,3,4 aDGlcpN 99.8/5.06 54.6/4.02 71.6/3.83 70.2/3.63 73.2/4.28 61.0/3.90-3.91
5,3,3 aDGalpA 102.5/5.47 69.6/3.93 72.2/4.26 80.9/4.48 72.5/4.67
5,3,7,7,4 aDGlcpN 95.6/5.33 55.1/3.34 70.4/4.02 76.6/3.60 73.2/4.19 60.0/3.90-3.91
5,3,7,7 aDGalpA 99.8/5.12 69.0/4.05 69.9/4.16 77.9/4.62 70.9/4.41
5,3,7 aXLDmanHepp 103.2/4.98 71.1/4.05 72.3/3.95 71.4/3.93 73.4/3.72 69.1/4.23 72.0/3.69-4.88
5,3 aXLDmanHepp 99.6/5.44 78.6/4.34 80.0/4.19 66.6/4.12 73.4/3.67 69.3/4.28 73.3/3.62-4.01
5,4 bDGlcp 103.3/4.51 73.9/3.36 76.2/3.55 69.9/3.56 76.2/3.55 61.5/3.87-3.95
5 aXLDmanHepp 101.7/5.19 71.3/4.15 74.7/4.25 74.4/4.35 72.2/4.26 69.4/4.18 63.9/3.80
aXKdo? 34.7/1.96-2.29 66.5/4.21 75.7/4.25 70.1/3.81 66.9/3.97 64.3/3.69-3.92
report error
Found 1 record.
Displayed record 1
]; meningitis [ICD11: 1D01 
iitd.pan.wroc.pl>