Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Enterobacter cloacae [ICD11:
XN3YM ]
NCBI PubMed ID: 28645003Publication DOI: 10.1016/j.carres.2017.05.021Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel
ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, 300071 Tianjin, China
An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C4115 and studied by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit of the O-polysaccharide was established: →2)-α-l-Rhap-(1→2)-α-l-Rhap-(1→2)-α-l-Rhap-(1→2)-α-d-Galp-(1→3)-α-d-FucpNAc-(1→. The O-antigen gene cluster of E. cloacae C4115 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in consistence with the O-polysaccharide structure. The O-antigen structure and gene cluster of E. cloacae C4115 are similar to those of E. cloacae G3421 studied by us earlier (Perepelov A.V. et al. Carbohydr. Res. 427 (2016) 55-59).
Lipopolysaccharide, O-antigen, bacterial polysaccharide structure, O-antigen gene cluster
Structure type: polymer chemical repeating unit
Location inside paper: p.112, chart 1 E. cloacae G3421
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_133754,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_158539,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, DNA sequencing, SDS-PAGE, sugar analysis, acid hydrolysis, GLC, GPC, delipidation, function analysis of gene clusters
Related record ID(s): 11950
NCBI Taxonomy refs (TaxIDs): 550Reference(s) to other database(s): GTC:G63289QL
Show glycosyltransferases
There is only one chemically distinct structure:
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