Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Bordetella pertussis [ICD11:
XN23B 
]
NCBI PubMed ID: 28122817Publication DOI: 10.1194/jlr.M072900Journal NLM ID: 0376606Publisher: ASBMB
Correspondence: martine.caroff
u-psud.fr
Institutions: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universite Paris-Sud, Universite Paris-Saclay, Orsay, France, LPS-BioSciences, Universite Paris-Sud, Orsay, France, L'Oreal, Centre de Recherches Biotechnologiques, 37390 Tours, France, Pediatric and Neonatal Intensive Care, Hopitaux Universitaires Paris-Sud, Assistance Publique-Hopitaux de Paris, 94275 Le Kremlin-Bicetre, France
Vitreoscilla filiformis is a Gram-negative bacterium isolated from spa waters and described for its beneficial effects on the skin. We characterized the detailed structure of its lipopolysaccharide (LPS) lipid A moiety, an active component of the bacterium that contributes to the observed skin activation properties. Two different batches differing in postculture cell recovery were tested. Chemical analyses and mass spectra, obtained before and after mild-alkali treatments, revealed that these lipids A share the common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxydecanoic acid in an amide linkage. Short-chain FAs, hydroxydecanoic and dodecanoic acid, were found in a 2:1 ratio. The two lipid A structures differed by the relative amount of the hexa-acyl molecular species and phosphoethanolamine substitution of the phosphate groups. The two V. filiformis LPS batches induced variable interleukin-6 and TNF-α secretion by stimulated myelomonocytic THP-1 cells, without any difference in reactive oxygen species production or activation of caspase 3/7. Other different well-known highly purified LPS samples were characterized structurally and used as standards. The structural data obtained in this work explain the low inflammatory response observed for V. filiformis LPS and the previously demonstrated beneficial effects on the skin.
Lipopolysaccharide, mass spectrometry, cytokines, Toll-like receptor, V. filiformis, lipid biochemistry, skin
Structure type: oligomer ; 1559.95 [M-H]-
Location inside paper: p.549, fig.4F, B. pertussis 1414
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_176772
Methods: GC-MS, SDS-PAGE, chemical analysis, ELISA, acid hydrolysis, GC, MALDI-MS, alkaline hydrolysis, UV, HPTLC, enzymatic treatments, cytokine production
Related record ID(s): 11951, 12197, 12198, 12199, 12200, 12201, 12202, 12204
NCBI Taxonomy refs (TaxIDs): 520
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There is only one chemically distinct structure:
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