Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperPublication DOI: 10.1007/s11172-017-1889-2Journal NLM ID: 7505544Publisher: Moscow: Nauka
Correspondence: yknirel
gmail.com; liubin1981
nankai.edu.cn
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Biological Sciences and Biotechnology, Tianjin Economic-Technological Development Area (TEDA), Nankai University, Tianjin, China
The structure of the O-specific polysaccharide (O-antigen) from Escherichia coli O92 was established. The acid-labile polysaccharide was produced by O-deacylation of the lipopolysaccharide under mild alkaline conditions. The methylation analysis combined with 1D and 2D 1H and 13C NMR spectroscopy demonstrated that the O-specific polysaccharide chain of the lipopolysaccharide is a linear fructan composed of mono-O-acetylated disaccharide repeating units having the structure →1)-b-d-Fruf3Ac-(2→3)-b-d-Fruf-(2→. To the best of our knowledge, this fructan has been hitherto unknown in bacteria.
Escherichia coli, ipopolysaccharide, O-antigen gene cluster, fructan, structure of the O-specific polysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: p.1305, table 1
Compound class: O-polysaccharide, O-antigen
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, acid hydrolysis, GC, mild alkaline degradation, GPC, bioinformatic analysis, mild acid degradation
Comments, role: E. coli O92 laboratory number G4216; O-deacetylated OPS.
Related record ID(s): 11960
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G36534HX
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3 bDFruf 62.8 104.6 78.0 74.7 82.4 62.2
bDFruf 63.3 105.4 78.9 75.9 81.8 63.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3 bDFruf 3.75-3.86 - 4.31 4.19 3.84 3.78-3.88
bDFruf 3.67-3.72 - 4.50 4.19 3.85 3.77-3.85
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3 bDFruf 62.8/3.75-3.86 78.0/4.31 74.7/4.19 82.4/3.84 62.2/3.78-3.88
bDFruf 63.3/3.67-3.72 78.9/4.50 75.9/4.19 81.8/3.85 63.2/3.77-3.85
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3 | bDFruf | 3.75
3.86 |
| 4.31 | 4.19 | 3.84 | 3.78
3.88 |
| | bDFruf | 3.67
3.72 |
| 4.50 | 4.19 | 3.85 | 3.77
3.85 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3 | bDFruf | 62.8 | 104.6 | 78.0 | 74.7 | 82.4 | 62.2 |
| | bDFruf | 63.3 | 105.4 | 78.9 | 75.9 | 81.8 | 63.2 |
|
There is only one chemically distinct structure:
Found 
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