Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Organ / tissue: gastrointestinal tract,
upper airwaysAssociated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
The structure was elucidated in this paperNCBI PubMed ID: 26723873Publication DOI: 10.1016/j.ijmm.2015.12.002Journal NLM ID: 100898849Publisher: Jena, Germany: Urban & Fischer Verlag
Correspondence: Gabor Nagy <gabor.nagy
arsanis.com>
Institutions: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Arsanis Biosciences GmbH, Vienna, Austria
Klebsiella pneumoniae ST258 is a globally disseminated, extremely drug resistant, nosocomial clone with limited treatment options. We show that the vast majority of ST258 isolates express modified d-galactan-I lipopolysaccharide O-antigen, termed hereinafter as D-galactan-III. The genetic determinant required for galactan-III synthesis was identified as a distinct operon adjacent to the rfb (wb) locus encoding D-galactan-I synthesis. The three genes within the operon encode predicted glycosyltransferases. Testing an isogenic transformant pair revealed that expression of D-galactan-III, in comparison to D-galactan-I, conferred improved survival in the presence of human serum. Eighty-three percent of the more than 200 ST258 draft genome sequences currently available carries the corresponding operon and hence these isolates are predicted to express galactan-III antigens. A D-galactan-III specific monoclonal antibody (mAb) was shown to bind to extracted LPS from a panel of ST258 isolates. The same mAb confirmed accessibility of galactan-III in surface staining of ST258 irrespective of the distinct capsular antigens expressed by both clades described previously. Based on these data, the galactan-III antigen may represent an attractive target for active and passive immunization approaches against K. pneumoniae ST258.
monoclonal antibody, LPS O-antigen, d-galactan-I, d-galactan-III, Klebsiella pneumoniae ST258, Serotype O2
Structure type: polymer chemical repeating unit
Location inside paper: table 2, Kp26, p.94, fig.4, PCM-27, Kp26, Kp26/pKP100, Kp26/pSU2718 D-galactan-I
Trivial name: D-galactan I, galactan I, galactan, D-galactan, microbial carbohydrate determinants, D-galactan-I
Compound class: CPS, EPS, O-polysaccharide, O-antigen, LPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
Methods: 13C NMR, 1H NMR, methylation, gel filtration, NMR-2D, sugar analysis, ELISA, mild acid hydrolysis, genetic methods, immunoblotting, serum bactericidal assays, HR-MAS NMR, statistical analysis, cloning, bioinformatic analysis, immunization, flow cytometry analysis
Related record ID(s): 11968
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G85324FU, GlycomeDB:
665
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3 bDGalf 110.2-110.5 80.5-81.4 85.4-85.6 80.5-82.6 71.0-71.5 63.5-63.6
aDGalp 100.1-100.4 67.9-68.6 77.5-78.0 70.0-70.1 72.1 61.91
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3 bDGalf 5.22 4.34-4.42 4.08 4.26-4.30 3.86-3.88 3.70-3.72
aDGalp 5.09-5.10 3.69-3.95 3.95-4.00 4.14-4.15 4.15 3.75
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3 bDGalf 110.2-110.5/5.22 80.5-81.4/4.34-4.42 85.4-85.6/4.08 80.5-82.6/4.26-4.30 71.0-71.5/3.86-3.88 63.5-63.6/3.70-3.72
aDGalp 100.1-100.4/5.09-5.10 67.9-68.6/3.69-3.95 77.5-78.0/3.95-4.00 70.0-70.1/4.14-4.15 72.1/4.15 61.91/3.75
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3 | bDGalf | 5.22 | 4.34
4.42 | 4.08 | 4.26
4.30 | 3.86
3.88 | 3.70
3.72 |
| | aDGalp | 5.09
5.10 | 3.69
3.95 | 3.95
4.00 | 4.14
4.15 | 4.15 | 3.75 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3 | bDGalf | 110.2
110.5 | 80.5
81.4 | 85.4
85.6 | 80.5
82.6 | 71.0
71.5 | 63.5
63.6 |
| | aDGalp | 100.1
100.4 | 67.9
68.6 | 77.5
78.0 | 70.0
70.1 | 72.1 | 61.91 |
|
There is only one chemically distinct structure:
Found 
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