Vinogradov E, Aubry A, Logan SM Structural characterization of wall and lipidated polysaccharides from Clostridium perfringens ATCC 13124 Carbohydrate Research448 (2017)
88-94
The structure was elucidated in this paper NCBI PubMed ID:28628892 Publication DOI:10.1016/j.carres.2017.06.003 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: Evguenii.Vinogradovnrc-cnrc.gc.ca Institutions: National Research Council of Canada, Vaccine Program, Human Health Therapeutics, Ottawa Canada, K1A OR6
Cell surface polysaccharides produced by C. perfringens ATCC 13124 were analyzed using NMR, chemical and immunological methods. Two distinct polymers were identified. The more abundant PS1 had a structure based on a polymer of β-mannosamine with a number of modifications, including varying levels of substitution at O-6 with PEtN, N-acetylation, and different linkages between monosaccharides. The shortest variant of PS1 represented a lipoteichoic acid. It contained only 1-4-linkages between ManNAc residues, minor branching α-Ribf, and glucosyl-glycerol at the reducing end, which was acylated with linear saturated fatty acids C16, C18, and C20 (dominant). Other non-lipidated variants of PS1 contained less PEtN, no α-Ribf, up to 50% 1-3-linkages, and up to 25% ManN with the free amino group. The minor polysaccharide PS2 had a linear regular structure with a -4-α-Rha-3-β-Gal-4-β-GalNAc3PCho- repeating unit, where PCho indicates phosphocholine.
Structure type: structural motif or average structure Location inside paper: p.92, fig.4, sPS1 Compound class: cell wall polysaccharide Contained glycoepitopes:IEDB_120354,IEDB_123890,IEDB_885813
Methods: 13C NMR, 1H NMR, NMR-2D, de-O-acylation, SDS-PAGE, sugar analysis, Western blotting, GPC, ion-exchange chromatography, HF treatment, N-acetylation, immunization, lysozyme treatment Comments, role: Possible structure of polysaccharide sPS1, the B-N-K ([-4)b?ManpNAc6PEtN(1-][-4)b?ManNAc(1-][-3)b?ManpNAc6PEtN(1-]) sequence was not proven.
Related record ID(s): 11976, 12255, 12256, 12257, 12259 NCBI Taxonomy refs (TaxIDs):195103 Reference(s) to other database(s): GTC:G51729VZ Show glycosyltransferases
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