Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 6360684Publication DOI: 10.1111/j.1432-1033.1983.tb07810.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-7800 Freiburg-Zähringen, Federal Republic of Germany
The O-specific polysaccharide moiety (04 polysaccharide) of the 04 antigen (lipopolysaccharide) from Escherichia coli 04:K12:H- was isolated in pure form by degradation of the lipopolysaccharide and chromatography on Sephadex G-50. The primary structure of the 04 polysaccharide was elucidated by composition, Smith degradation, nuclear magnetic resonance spectroscopy, methylation and oligosaccharide analysis. Oligosaccharides were obtained by deaminating fragmentation of partially and completely de-N-acetylated polysaccharides. The polysaccharide consists of repeating units of the pentasaccharide (formula; see text) which are joined in the polymer through α-1,4-linkages. The 04 polysaccharide has a mean molecular mass of 13800 Da and consists of the core oligosaccharide and about 14 pentasaccharide repeating units.
Structure type: polymer chemical repeating unit ; n=14
Location inside paper: abstract
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_143253,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_158539,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GC-MS
NCBI Taxonomy refs (TaxIDs): 83333Reference(s) to other database(s): GTC:G09548UE, GlycomeDB:
7747
Show glycosyltransferases
There is only one chemically distinct structure: