Romanowska E, Gamian A, Dabrowski J Core region of Citrobacter lipopolysaccharide from strain PCM 1487. Structure elucidation by two-dimensional proton NMR spectroscopy at 500-MHz and methylation analysis/mass spectrometry European Journal of Biochemistry161 (1986)
557-564
The structure was elucidated in this paper NCBI PubMed ID:3024976 Journal NLM ID:0107600 Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
The core structure of Citrobacter PCM 1487 lipopolysaccharide has been established using methylation analysis/mass spectrometry, chemical degradations and one- and two-dimensional 1H-NMR spectroscopy at 500 MHz. 1H-NMR assignments are given for all sugar components of the core oligosaccharide. In the formula shown below, the alternative locations of branch terminal heptose (LDHep) and diphosphorylethanolamine (PPEtN) residues are marked by dashed lines; dOclA stands for 3-deoxy-D-manno-octulosonic acid. (Formula: see text). The sample of the core oligosaccharide showed some microheterogeneity due to a slightly incomplete substitution by terminal N-acetylgalactosamine and a partial splitting of diphosphorylethanolamine residues.
Methods: 1H NMR, GC-MS Comments, role: partial structure, additional L-glycero-D-manno-heptose and diphosphoetanolamine groups are attached to the hepotose region at unknown positions
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