Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: dysentery [ICD11:
SA56 
];
diarrhea [ICD11:
ME05.1 , ICD11:
SA55 ];
gastroenteritis [ICD11:
1A40.0 ];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
The structure was elucidated in this paperNCBI PubMed ID: 29186063Publication DOI: 10.3390/ijms18122572Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: tomasz.niedziela
iitd.pan.wroc.pl
Institutions: Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland
The high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR) analysis of Plesiomonas shigelloides 78/89 lipopolysaccharide directly on bacteria revealed the characteristic structural features of the O-acetylated polysaccharide in the NMR spectra. The O-antigen profiles were unique, yet the pattern of signals in the, spectra along with their (1)H,13C chemical shift values, resembled these of d-galactan I of Klebsiella pneumoniae. The isolated O-specific polysaccharide (O-PS) of P. shigelloides strain CNCTC 78/89 was investigated by 1H and 13C NMR spectroscopy, mass spectrometry and chemical methods. The analyses demonstrated that the P. shigelloides 78/89 O-PS is composed of →3)-α-d-Galp-(1→3)-β-d-Galf2OAc-(1→ disaccharide repeating units. The O-acetylation was incomplete and resulted in a microheterogeneity of the O-antigen. This O-acetylation generates additional antigenic determinants within the O-antigen, forms a new chemotype, and contributes to the epitopes recognized by the O-serotype specific antibodies. The serological cross-reactivities further confirmed the inter-specific structural similarity of these O-antigens.
Lipopolysaccharide, O-antigen, NMR spectroscopy, O-acetylation, HR-MAS, Plesiomonas shigelloides, d-galactan I
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1
Trivial name: D-galactan I, galactan I, galactan, D-galactan, microbial carbohydrate determinants, D-galactan-I
Compound class: CPS, EPS, O-polysaccharide, O-antigen, LPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
Methods: 13C NMR, 1H NMR, gel filtration, SDS-PAGE, sugar analysis, ELISA, mild acid hydrolysis, MALDI-TOF MS, immunoblotting, HR-MAS NMR, acetylation
Comments, role: non-O-acetylated glycoform of OPS; NMR data for non-reducing terminal residue aDGalp 1H: 5.11 3.90 4.01 4.12 4.27 3.75-3.8, 13C: 95.4 68.5 69.6 70.3 71.2 60.7.
Related record ID(s): 11995
NCBI Taxonomy refs (TaxIDs): 703,
573Reference(s) to other database(s): GTC:G85324FU, GlycomeDB:
665
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3 bDGalf 109.4 79.8 84.5 81.8 70.8 62.7
aDGalp 99.5 67.2 76.9 69.2 71.3 61.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3 bDGalf 5.20 4.40 4.06 4.24 3.86 3.66-3.68
aDGalp 5.07 3.93 3.93 4.12 4.12 3.68-3.73
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3 bDGalf 109.4/5.20 79.8/4.40 84.5/4.06 81.8/4.24 70.8/3.86 62.7/3.66-3.68
aDGalp 99.5/5.07 67.2/3.93 76.9/3.93 69.2/4.12 71.3/4.12 61.1/3.68-3.73
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3 | bDGalf | 5.20 | 4.40 | 4.06 | 4.24 | 3.86 | 3.66
3.68 |
| | aDGalp | 5.07 | 3.93 | 3.93 | 4.12 | 4.12 | 3.68
3.73 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3 | bDGalf | 109.4 | 79.8 | 84.5 | 81.8 | 70.8 | 62.7 |
| | aDGalp | 99.5 | 67.2 | 76.9 | 69.2 | 71.3 | 61.1 |
|
There is only one chemically distinct structure:
Found 
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