Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia [ICD11:
XN01M 
]
The structure was elucidated in this paperNCBI PubMed ID: 28972189Publication DOI: 10.1074/jbc.M117.813832Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: brlee
pusan.ac.kr
Institutions: Department of Microbiology, Kosin University College of Medicine, Busan 49267, South Korea, Global Research Laboratory, College of Pharmacy, Pusan National University, Busan 46241, South Korea, Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, Napoli 80126, Italy, CNR-Istituto per i Polimeri, Compositi e Biomateriali IPCB, Via P. Gaifami 18, Catania 95126, Italy
Lipopolysaccharide, the outer cell-wall component of Gram-negative bacteria, has been shown to be important for symbiotic associations. We recently reported that the lipopolysaccharide O-antigen of Burkholderia enhances the initial colonization of the midgut of the bean bug, Riptortus pedestris However, the midgut-colonizing Burkholderia symbionts lack the O-antigen but display the core oligosaccharide on the cell surface. In this study, we investigated the role of the core oligosaccharide, which directly interacts with the host midgut, in the Riptortus-Burkholderia symbiosis. To this end, we generated the core oligosaccharide mutant strains, ΔwabS, ΔwabO, ΔwaaF, and ΔwaaC, and determined the chemical structures of their oligosaccharides, which exhibited different compositions. The symbiotic properties of these mutant strains were compared with those of the wild-type and O-antigen-deficient ΔwbiG strains. Upon introduction into Riptortus via the oral route, the core oligosaccharide mutant strains exhibited different rates of colonization of the insect midgut. The symbiont titers in fifth-instar insects revealed significantly reduced population sizes of the inner core oligosaccharide mutant strains ΔwaaF and ΔwaaC These two strains also negatively affected host growth rate and fitness. Furthermore, R. pedestris individuals colonized with the ΔwaaF and ΔwaaC strains were vulnerable to septic bacterial challenge, similar to insects without a Burkholderia symbiont. Taken together, these results suggest that the core oligosaccharide from Burkholderia symbionts plays a critical role in maintaining a proper symbiont population and in supporting the beneficial effects of the symbiont on its host in the Riptortus-Burkholderia symbiosis.
Lipopolysaccharide, biosynthesis, core oligosaccharide, insect symbiosis, symbiotic role, gut symbiont
Structure type: oligomer
Location inside paper: fig.3B, ΔwabO
Aglycon: (2->?)lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_135607,IEDB_135609,IEDB_140088,IEDB_2189047
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, sugar analysis, mild acid hydrolysis, MALDI-TOF MS, genetic methods, GPC, enzymatic digestion, statistical analysis, SDS-Tricine-PAGE, motility assays, symbiont titers assays
Related record ID(s): 12031, 12418, 12420, 12421
NCBI Taxonomy refs (TaxIDs): 1317920Reference(s) to other database(s): GTC:G14667PH
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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