The structure was elucidated in this paper NCBI PubMed ID:29660546 Publication DOI:10.1016/j.carres.2018.04.001 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: A.V. Perepelov <andreivperepelovgmail.com> Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China, Russian Federation, School of Basic Medical Sciences, Tianjin Medical University, Heping District, Tianjin, 300070, PR China
Mild acid hydrolysis of the lipopolysaccharide of Escherichia coli O54 afforded an O-polysaccharide, which was studied by sugar analysis, solvolysis with anhydrous trifluoroacetic acid, and 1H and 13C NMR spectroscopy. Solvolysis cleaved predominantly the linkage of β-d-Ribf and, to a lesser extent, that of β-d-GlcpNAc, whereas the other linkages, including the linkage of α-l-Rhap, were stable under selected conditions (40 °C, 5 h). The following structure of the O-polysaccharide was established: →4)-α-d-GalpA-(1 → 2)-α-l-Rhap-(1 → 2)-β-d-Ribf-(1 → 4)-β-d-Galp-(1 → 3)-β-d-GlcpNAc-(1→ The O-antigen gene cluster of E. coli O54 was analyzed and found to be consistent in general with the O-polysaccharide structure established but there were two exceptions: i) in the cluster, there were genes for phosphoserine phosphatase and serine transferase, which have no apparent role in the O-polysaccharide synthesis, and ii) no ribofuranosyltransferase gene was present in the cluster. Both uncommon features are shared by some other enteric bacteria.
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ESI-MS, GLC, GPC, acetylation, delipidation, function analysis of gene clusters, solvolysis with trifluoroacetic acid Comments, role: E. coli O54 (laboratory stock number G3091); oligosaccharide derived from the OPS by solvolysis with CF3CO2H.
Related record ID(s): 12457, 12458, 12462 NCBI Taxonomy refs (TaxIDs):2184075 Reference(s) to other database(s): GTC:G29294HT Show glycosyltransferases
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