Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Sus scrofa; Homo sapiens
Associated disease: meningitis [ICD11:
1D01 
];
septicemia [ICD11:
MA15.Y ];
inflammatory arthropathies [ICD11:
FA2Z ];
infection due to Streptococcus suis [ICD11:
XN5SE ]
The structure was elucidated in this paperNCBI PubMed ID: 29884769Publication DOI: 10.1074/jbc.RA118.002174Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: ngisch
fz-borstel.de
Institutions: Division of Bioanalytical Chemistry, Priority Area Infections, Research Center Borstel, Leibniz Lung Center, 23845 Borstel, Germany, Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec J2S 2M2, Canada, the State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Streptococcus suis serotype 2 is an important porcine and human pathogen. Lipoteichoic acid (LTA) from S. suis has been suggested to contribute to its virulence, and absence of d-alanylation from the S. suis LTA is associated with increased susceptibility to cationic antimicrobial peptides. Here, using high-resolution NMR spectroscopy and MS analyses, we characterized the LTA structures from three S. suis serotype 2 strains differing in virulence, sequence type (ST), and geographical origin. Our analyses revealed that these strains possess–in addition to the typical type I LTA present in other streptococci–a second, mixed-type series of LTA molecules of high complexity. We observed a ST-specific difference in the incorporation of glycosyl residues into these mixed-type LTAs. We found that strains P1/7 (ST1, high virulence) and SC84 (ST7, very high virulence) can attach a 1,2-linked α-D-Glcp residue as branching substituent to an α-D-Glcp that is 1,3-linked to glycerol phosphate moieties and that is not present in strain 89-1591 (ST25, intermediate virulence). In contrast, the latter strain could glycosylate its LTA at the glycerol O-2 position, which was not observed in the other two strains. Using LTA preparations from WT strains and from mutants with an inactivated prolipoprotein diacylglyceryl transferase, resulting in deficient lipoprotein acylation, we show that S. suis LTAs alone do not induce Toll-like receptor 2–dependent pro-inflammatory mediator production from dendritic cells. In summary, our study reveals an unexpected complexity of LTAs present in three S. suis serotype 2 strains differing in genetic background and virulence.
NMR, bacteria, cell wall, mass spectrometry, lipoteichoic acid, lipoprotein, Streptococcus suis, bacterial virulence, glycolipid structure
Structure type: oligomer
Location inside paper: fig.2 compound 5 of S. suis 89-1591 (ST25), table S5
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, DNA techniques, 31P NMR, de-O-acylation with hydrazine, composition analysis, GPC, extraction, HF treatment, CID-MS, statistical analysis
Comments, role: repeating unit LTA after HF treatment; Streptococcus suis serotype 2 strain 89-1591(ST25)
Related record ID(s): 12563, 12564, 12565, 12566, 12567, 12568, 12569, 12570, 12571, 12572, 12573, 12575, 12576, 12904
NCBI Taxonomy refs (TaxIDs): 1214151Reference(s) to other database(s): GTC:G01348VS
Show glycosyltransferases
NMR conditions: in D2O; pH 5.5 at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
1 aDGlcp 99.3 72.0 73.6 70.2 72.5 61.0
2 aDGlcp 98.4 72.0 73.5 70.2 72.6 61.1
x?Gro 67.4 77.2 62.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
1 aDGlcp 4.95 3.54-3.57 3.69-3.74 3.39-3.43 3.68-3.74 3.69-3.88
2 aDGlcp 5.19 3.54-3.58 3.72-3.76 3.40-3.44 3.81-3.85 3.73-3.85
x?Gro 3.62-3.94 3.98-4.02 3.72-3.80
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
1 aDGlcp 99.3/4.95 72.0/3.54-3.57 73.6/3.69-3.74 70.2/3.39-3.43 72.5/3.68-3.74 61.0/3.69-3.88
2 aDGlcp 98.4/5.19 72.0/3.54-3.58 73.5/3.72-3.76 70.2/3.40-3.44 72.6/3.81-3.85 61.1/3.73-3.85
x?Gro 67.4/3.62-3.94 77.2/3.98-4.02 62.2/3.72-3.80
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 1 | aDGlcp | 4.95 | 3.54
3.57 | 3.69
3.74 | 3.39
3.43 | 3.68
3.74 | 3.69
3.88 |
| 2 | aDGlcp | 5.19 | 3.54
3.58 | 3.72
3.76 | 3.40
3.44 | 3.81
3.85 | 3.73
3.85 |
| | x?Gro | 3.62
3.94 | 3.98
4.02 | 3.72
3.80 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 1 | aDGlcp | 99.3 | 72.0 | 73.6 | 70.2 | 72.5 | 61.0 |
| 2 | aDGlcp | 98.4 | 72.0 | 73.5 | 70.2 | 72.6 | 61.1 |
| | x?Gro | 67.4 | 77.2 | 62.2 | |
|
There is only one chemically distinct structure:
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