Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperNCBI PubMed ID: 30200867Publication DOI: 10.1134/S0006297918070064Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: yknirel
gmail.com; nastia-kasimova979797
mail.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia, School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia, Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD 4059, Australia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141700, Russia, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Type K82 capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii LUH5534. The structure of a linear tetrasaccharide repeating unit of the CPS was established by sugar analysis along with one- and two-dimensional 1H and 13C NMR spectroscopy. Proteins encoded by the KL82 capsule gene cluster in the genome of LUH5534 were assigned to roles in the synthesis of the K82 CPS. In particular, functions were assigned to two new glycosyltransferases (Gtr152 and Gtr153) and a novel pyruvyltransferase, Ptr5, responsible for the synthesis of d-galactose 4,6-(R)-pyruvic acid acetal.
Acinetobacter baumannii, capsular polysaccharide structure, biotechnology, Pyruvic acid acetal, K locus, genetics of capsule biosynthesis
Structure type: polymer chemical repeating unit
Location inside paper: p.833, fig.2, MPS
Trivial name: type K82 CPS
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_134627,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_142078,IEDB_147450,IEDB_150899,IEDB_151531,IEDB_153216,IEDB_190606,SB_137,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_29,SB_7,SB_8,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, mild acid hydrolysis, GPC, dialysis, bioinformatic analysis (BLASTp)
Comments, role: depyruvylated CPS
Related record ID(s): 12918
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G50262AY
Show glycosyltransferases
NMR conditions: in D2O at 333 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3 bDGalp 101.8 79.5 75.0 70.1 76.4 62.4
3,3,2 Ac 175.2-176.0 23.4-23.6
3,3 aDGlcpN 95.5 53.6 80.1 69.2 69.9 62.4
3 bDGalp 106.1 70.4 78.6 66.3 76.1 62.3
2 Ac 175.2-176.0 23.4-23.6
bDGalpN 103.1 52.6 82.2 69.6 76.1 61.6
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3 bDGalp 4.59 3.66 3.69 3.86 3.69 3.73
3,3,2 Ac - 2.01-2.13
3,3 aDGlcpN 5.02 4.11 4.08 3.66 4.00 3.73-3.82
3 bDGalp 4.47 3.62 3.67 4.06 3.60 3.75
2 Ac - 2.01-2.13
bDGalpN 4.77 4.03 3.82 4.15 3.64 3.82
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3 bDGalp 101.8/4.59 79.5/3.66 75.0/3.69 70.1/3.86 76.4/3.69 62.4/3.73
3,3,2 Ac 23.4-23.6/2.01-2.13
3,3 aDGlcpN 95.5/5.02 53.6/4.11 80.1/4.08 69.2/3.66 69.9/4.00 62.4/3.73-3.82
3 bDGalp 106.1/4.47 70.4/3.62 78.6/3.67 66.3/4.06 76.1/3.60 62.3/3.75
2 Ac 23.4-23.6/2.01-2.13
bDGalpN 103.1/4.77 52.6/4.03 82.2/3.82 69.6/4.15 76.1/3.64 61.6/3.82
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3 | bDGalp | 4.59 | 3.66 | 3.69 | 3.86 | 3.69 | 3.73 |
| 3,3,2 | Ac |
| 2.01
2.13 | |
| 3,3 | aDGlcpN | 5.02 | 4.11 | 4.08 | 3.66 | 4.00 | 3.73
3.82 |
| 3 | bDGalp | 4.47 | 3.62 | 3.67 | 4.06 | 3.60 | 3.75 |
| 2 | Ac |
| 2.01
2.13 | |
| | bDGalpN | 4.77 | 4.03 | 3.82 | 4.15 | 3.64 | 3.82 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3 | bDGalp | 101.8 | 79.5 | 75.0 | 70.1 | 76.4 | 62.4 |
| 3,3,2 | Ac | 175.2
176.0 | 23.4
23.6 | |
| 3,3 | aDGlcpN | 95.5 | 53.6 | 80.1 | 69.2 | 69.9 | 62.4 |
| 3 | bDGalp | 106.1 | 70.4 | 78.6 | 66.3 | 76.1 | 62.3 |
| 2 | Ac | 175.2
176.0 | 23.4
23.6 | |
| | bDGalpN | 103.1 | 52.6 | 82.2 | 69.6 | 76.1 | 61.6 |
|
There is only one chemically distinct structure:
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