Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 29864581Publication DOI: 10.1016/j.carres.2018.05.006Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: andreivperepelov
gmail.com
Institutions: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47, Leninsky Prosp., Moscow, Russian Federation, Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russian Federation
Mild acid hydrolysis of the lipopolysaccharide of Escherichia coli O57 afforded an O-polysaccharide, which was isolated by gel permeation chromatography (GPC) and studied by sugar analysis, Smith degradation and solvolysis with trifluoroacetic acid, along with 2D 1H and 13C NMR spectroscopy. The O-polysaccharide was found to contain d-Glc, d-Gal, d-GalA, d-GlcNAc, and l-FucNAc, as well as O-acetyl groups. Smith degradation of the O-deacetylated polysaccharide destroyed side-branch β-Glсp and α-GalpA to give a modified linear polysaccharide. Solvolysis cleaved selectively the linkage of α-l-FucpNAc to give a pentasaccharide corresponding to the O-polysaccharide repeat. A comparison of the NMR spectra of the initial and O-deacetylated polysaccharides showed that α-GalpA is non-stoichiometrically O-acetylated at position either 2 (~30%) or 3 (~40%). The following structure of the O-polysaccharide was established, which is unique among known bacterial polysaccharide structures.
O-antigen, Escherichia coli, O-polysaccharide, bacterial polysaccharide structure, CF3CO2H solvolysis
Structure type: oligomer
Location inside paper: abstract, chart 1, p.3, structure OS1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ESI-MS, GLC, mild acid hydrolysis, Smith degradation, de-O-acetylation, GPC, delipidation, solvolysis with trifluoroacetic acid
Comments, role: OS1 was obtained by CF3CO2H solvolysis of O-polysaccharide (PS1)
Related record ID(s): 12731, 12732, 12734, 12735, 12951
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G73267DX
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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