Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 29524727Publication DOI: 10.1016/j.carres.2018.02.014Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Y.A. Knirel <knirel
ioc.ac.ru>; S.V. Dentovskaya <dentovskaya
obolensk.org>
Institutions: State Research Center for Applied Microbiology and Biotechnology, 142279, Obolensk, Moscow Region, Russia, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, Moscow, Russia
Lipopolysaccharide was isolated from bacteria Yersinia intermedia H9-36/83 (O:17) and degraded with mild acid to give an O-specific polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar analysis and 1D and 2D NMR spectroscopy. The polysaccharide was found to contain 3-deoxy-3-[(R)-3-hydroxybutanoylamino]-d-fucose (d-Fuc3NR3Hb) and the following structure of the heptasaccharide repeating unit was established: The structure established is consistent with the gene content of the O-antigen gene cluster. The O-polysaccharide structure and gene cluster of Y. intermedia are related to those of Hafnia alvei 1211 and Escherichia coli O:103.
Lipopolysaccharide, O-antigen, gene cluster, O-specific polysaccharide, Bacterial polysaccharide, 3-Amino-3-deoxy-d-fucose, Yersinia intermedia
Structure type: suggested polymer biological repeating unit
Location inside paper: p.55, chart 1, E. coli O103
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_885822,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, GPC, mild acid degradation, function analysis of gene clusters
Comments, role: structure from ref. [12]
Related record ID(s): 12797, 12983
NCBI Taxonomy refs (TaxIDs): 1055536
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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