Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 29304442Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E.L. Zdorovenko <zdorovenkoe
mail.ru>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 220141 Minsk, Belarus, Institute of Microbiology, National Academy of Sciences of Belarus, 220141 Minsk, Belarus
Two specific polysaccharides, together with an →4)-α-d-Glcp-(1→ glucan (bacterial glycogen), were obtained from a lipopolysaccharide preparation isolated from the bacterium Pseudomonas putida BIM B-1100 by phenol/water extraction. The following structures of the polysaccharides were established by composition analysis, Smith degradation, ESI-MS, and 1D and 2D NMR spectroscopy.
Lipopolysaccharide, O-polysaccharide, bacterial polysaccharide structure, Pseudomonas putida
Structure type: homopolymer
Location inside paper: table 1, p.12, chart 1, DPSIII, chart 2, PSIII
Trivial name: methyl glucose lipopolysaccharide, glucan, maltosaccharide, α-1,4-D-glucan, amylose, α-glucan, glycogen backbone, α-(1,4)-glucan, starch, α-(1-4)-glucan, starch, glycogen
Compound class: CPS, EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, methyl glucose lipopolysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, ESI-MS, GLC, Smith degradation, de-O-acetylation, composition analysis, GPC, mild acid degradation
Comments, role: O-deacetylated PS (DPSIII) and PSIII
Related record ID(s): 12842, 12843, 12845, 12846, 12847, 13008
NCBI Taxonomy refs (TaxIDs): 303Reference(s) to other database(s): GTC:G05740LL, GlycomeDB:
12100, CCSD:
4943, CBank-STR:819
Show glycosyltransferases
NMR conditions: in D2O at 323 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 101.3 73.17 74.7 78.8 72.7 62.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 5.35 3.59 3.96 3.63 3.82 3.79-3.87
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 101.3/5.35 73.17/3.59 74.7/3.96 78.8/3.63 72.7/3.82 62.0/3.79-3.87
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 5.35 | 3.59 | 3.96 | 3.63 | 3.82 | 3.79
3.87 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 101.3 | 73.17 | 74.7 | 78.8 | 72.7 | 62.0 |
|
There is only one chemically distinct structure:
Found 
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