Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperNCBI PubMed ID: 29300154Publication DOI: 10.1099/mic.0.000598Journal NLM ID: 0376646Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
Correspondence: Anastasiya A. Kasimova <nastia-kasimova979797
mail.ru>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia, School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia, Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia
The structures of capsular polysaccharides (CPSs) produced by different Acinetobacter baumannii strains have proven to be invaluable in confirming the role of specific genes in the synthesis of rare sugars through the correlation of genetic content at the CPS biosynthesis locus with sugars found in corresponding CPS structures. A module of four genes (rmlA, rmlB, vioA and vioB) was identified in the KL57 capsule biosynthesis gene cluster of A. baumannii isolate BAL_212 from Vietnam. These genes were predicted to direct the synthesis of 4-acetamido-4,6-dideoxy-d-glucose (N-acetylviosamine, d-Qui4NAc) and the K57 CPS was found to contain this monosaccharide. The K57 structure was determined and, in addition to d-Qui4NAc, included three N-acetylgalactosamine residues in the main chain, with a single glucose side branch. The KL57 gene cluster has not been found in any other A. baumannii genomes, but the rmlA-rmlB-vioA-vioB module is present in the KL119 gene cluster that would likely produce a d-Qui4NAc-containing CPS.
Acinetobacter baumannii, capsule, amino, K locus, K57, N-acetylviosamine
Structure type: polymer chemical repeating unit
Location inside paper: p.219, fig.3, table S1, K57 CPS
Trivial name: type K57 CPS
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141582,IEDB_141584,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_885822,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, GLC, GPC, function analysis of gene clusters
Biosynthesis and genetic data: Wzy(K57), Gtr115, Gtr116, Gtr117, Gtr50, Wzy(K57)[ItrA2](transferases)
Comments, role: Acinetobacter baumannii isolate BAL_212 from Vietnam; the structure of the K57 CPS was determined as described in ref. [5].
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G82786KR, GlycomeDB:
25210, SpecDB: ol.9
Show glycosyltransferases
NMR conditions: in D2O at 333 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,3,4 Ac 175.0-175.9 23.3-23.7
3,4,3 bDQuip4N 105.2 73.6 79.4 57.6 72.2 17.9
3,4,2 Ac 175.0-175.9 23.3-23.7
3,4,6 aDGlcp 99.9 72.7 74.4 70.9 73.3 62.0
3,4 aDGalpN 99.6 50.0 78.2 69.4 69.9 67.3
3,2 Ac 175.0-175.9 23.3-23.7
3 aDGalpN 94.9 51.1 68.4 77.5 71.4 62.0
2 Ac 175.0-175.9 23.3-23.7
aDGalpN 98.9 49.0 73.9 65.7 73.4 61.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3,4 Ac - 2.03-2.06
3,4,3 bDQuip4N 4.58 3.43 3.72 3.77 3.55 1.17
3,4,2 Ac - 2.03-2.06
3,4,6 aDGlcp 4.91 3.52 3.67 3.39 3.63 3.73-3.82
3,4 aDGalpN 4.94 4.40 4.12 4.29 4.56 3.67-3.78
3,2 Ac - 2.03-2.06
3 aDGalpN 5.14 4.29 3.87 4.08 3.88 3.72
2 Ac - 2.03-2.06
aDGalpN 5.08 4.41 2.98 4.19 4.33 3.73
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,3,4 Ac 23.3-23.7/2.03-2.06
3,4,3 bDQuip4N 105.2/4.58 73.6/3.43 79.4/3.72 57.6/3.77 72.2/3.55 17.9/1.17
3,4,2 Ac 23.3-23.7/2.03-2.06
3,4,6 aDGlcp 99.9/4.91 72.7/3.52 74.4/3.67 70.9/3.39 73.3/3.63 62.0/3.73-3.82
3,4 aDGalpN 99.6/4.94 50.0/4.40 78.2/4.12 69.4/4.29 69.9/4.56 67.3/3.67-3.78
3,2 Ac 23.3-23.7/2.03-2.06
3 aDGalpN 94.9/5.14 51.1/4.29 68.4/3.87 77.5/4.08 71.4/3.88 62.0/3.72
2 Ac 23.3-23.7/2.03-2.06
aDGalpN 98.9/5.08 49.0/4.41 73.9/2.98 65.7/4.19 73.4/4.33 61.5/3.73
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3,4 | Ac |
| 2.03
2.06 | |
| 3,4,3 | bDQuip4N | 4.58 | 3.43 | 3.72 | 3.77 | 3.55 | 1.17 |
| 3,4,2 | Ac |
| 2.03
2.06 | |
| 3,4,6 | aDGlcp | 4.91 | 3.52 | 3.67 | 3.39 | 3.63 | 3.73
3.82 |
| 3,4 | aDGalpN | 4.94 | 4.40 | 4.12 | 4.29 | 4.56 | 3.67
3.78 |
| 3,2 | Ac |
| 2.03
2.06 | |
| 3 | aDGalpN | 5.14 | 4.29 | 3.87 | 4.08 | 3.88 | 3.72 |
| 2 | Ac |
| 2.03
2.06 | |
| | aDGalpN | 5.08 | 4.41 | 2.98 | 4.19 | 4.33 | 3.73 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,3,4 | Ac | 175.0
175.9 | 23.3
23.7 | |
| 3,4,3 | bDQuip4N | 105.2 | 73.6 | 79.4 | 57.6 | 72.2 | 17.9 |
| 3,4,2 | Ac | 175.0
175.9 | 23.3
23.7 | |
| 3,4,6 | aDGlcp | 99.9 | 72.7 | 74.4 | 70.9 | 73.3 | 62.0 |
| 3,4 | aDGalpN | 99.6 | 50.0 | 78.2 | 69.4 | 69.9 | 67.3 |
| 3,2 | Ac | 175.0
175.9 | 23.3
23.7 | |
| 3 | aDGalpN | 94.9 | 51.1 | 68.4 | 77.5 | 71.4 | 62.0 |
| 2 | Ac | 175.0
175.9 | 23.3
23.7 | |
| | aDGalpN | 98.9 | 49.0 | 73.9 | 65.7 | 73.4 | 61.5 |
|
There is only one chemically distinct structure:
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