Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Homo sapiens
Associated disease: pneumonia [ICD11:
CA40 
];
infection due to Streptococcus pneumoniae [ICD11:
XN3PW ]
NCBI PubMed ID: 29715685Publication DOI: 10.1016/j.carres.2018.04.011Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: jduus
kemi.dtu.dk
Institutions: Department of Chemistry, Technical University of Denmark, Kgs. Lyngby, Denmark, SSI Diagnostica A/S, Hilleroed, Denmark, Respiratory and Vaccine Preventable Bacterial Reference Unit, Public Health England - National Infection Service, Colindale, London, UK
Streptococcus pneumoniae is characterised into 92 serotypes based on antigenic reactions of commercial rabbit sera to the capsular polysaccharides. During development of a bioinformatic serotyping tool (PneumoCaT), an isolate exhibited a novel codon at residue 385 of the glycosyltransferase gene wcwK encoding a distinct amino acid, which differentiates genogroup 7. Investigation by repeat serotyping and Quellung reaction revealed a novel pattern of factor sera with the isolate reacting very strongly with 7f, but also with 7e factor sera. The structure of the capsular polysaccharide was determined by NMR spectroscopy to be an approximately 5:1 combination of the structures of 7C and 7B, respectively, and the structure of 7C was also elucidated. All data from whole genome sequencing, NMR spectroscopy, production of antisera and serotyping of the novel 7 strain shows that it is a new serotype, which will be named in the Danish nomenclature as 7D.
Streptococcus pneumoniae, NMR spectroscopy, structure elucidation, Hybrid serotype, Whole genome sequence
Structure type: polymer biological repeating unit
Location inside paper: p.25, fig.1
Trivial name: teichoic acid
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_114703,IEDB_115009,IEDB_116046,IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_140624,IEDB_141109,IEDB_141582,IEDB_141584,IEDB_142345,IEDB_142488,IEDB_146664,IEDB_153207,IEDB_241118,IEDB_591403,IEDB_885822,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, DNA sequencing, 31P NMR, bioinformatic analysis, serotyping, DNA extraction
Related record ID(s): 12636, 12637, 12638, 12639
NCBI Taxonomy refs (TaxIDs): 1313
Show glycosyltransferases
There is only one chemically distinct structure:
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