Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
NCBI PubMed ID: 29965749Publication DOI: 10.1021/jacs.8b04078Journal NLM ID: 7503056Publisher: American Chemical Society
Correspondence: shwu
gate.sinica.edu.tw; cyiwu
gate.sinica.edu.tw
Institutions: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan, Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan, Graduate Institute of Life Science, National Defense Medical Center, Taipei 112, Taiwan, Department of Microbiology , Tzu Chi University, Hualien 970, Taiwan, Department of Chemistry , National Taiwan University, Taipei 106, Taiwan
Pseudaminic acid (Pse) has been known for participating in crucial bacterial virulence and thus is an attractive target in the development of glycoconjugate vaccine. Particularly, this therapeutic alternative was suggested to be a potential solution against antibiotic resistant Acinetobacter baumannii that poses a serious global health threat. Also, Pse was found to be involved in the exopolysaccharide (EPS) of mild antibiotic resistant Acinetobacter baumannii strain 54149 (Ab-54149) of which specific glycosyl linkage can be depolymerized by phagePhiAB6 tailspike protein (PhiAB6TSP). In this study, we found that the antibodies induced by Ab-54149 EPS was capable of recognizing a range of EPS of other clinical A. baumannii strains, and deemed as a great potential material for vaccination. To efficiently acquire homogeneous EPS-derived oligosaccharide with significant immunogenic activity for the production of glycoconjugate, we used the PhiAB6TSP for the fragmentation of Ab-54149 EPS instead of chemical methods. Moreover, insight into the ligand binding characterization of PhiAB6TSP suggested the branched Pse on the Ab-54149 EPS served as a recognition site of PhiAB6TSP. The serum boosted by PhiAB6TSP-digested product and carrier protein CRM197 conjugate complex displayed specific sensitivity toward Ab-54149 EPS with bacterial killing activity. Strikingly, Pse is an ideal epitope with strong antigenicity, profiting the application of the probe for pathogen detection and glyco-based vaccine.
Acinetobacter baumannii, pseudaminic acid, exopolysaccharide, vaccine, CRM197, glycoconjugate vaccine
Structure type: polymer chemical repeating unit
Location inside paper: p.8640, fig.S1B, fig.S4
Compound class: EPS
Contained glycoepitopes: IEDB_130648,IEDB_134627,IEDB_136044,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_147450,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_23,SB_24,SB_7,SB_8,SB_88
Methods: 13C NMR, 1H NMR, dot immunoblotting, ESI-MS, ESI-MS/MS, MALDI-TOF MS, STD NMR, extraction, serum bactericidal assays, conjugation, flow cytometry analysis, enzyme kinetic assay, bacteriophage digestion, substrate binding
Comments, role: major repeat units (~ 90%)
3D data: the FAB6TSP substrat complex structure
Related record ID(s): 12651, 12652
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G74492VS
Show glycosyltransferases
[as TSV]
1H NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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