Department of Biotechnology and Molecular Biology, University of Opole, PL-Opole, Poland, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Laboratory of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL-90-237 Lodz, Poland, Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, 90-237 Lodz, Poland
The new type of core oligosaccharide in Proteus penneri 40A and 41 lipopolysaccharides has been investigated by 1H and 13C NMR spectroscopy, electrospray ionization mass spectrometry and chemical methods. Core oligosaccharides of both strains were chosen for structural analysis based on the reactivity of LPSs with serum against P. penneri 40A core oligosaccharide-diphtheria toxoid conjugate. Structural analyses revealed that P. penneri 40A and 41 LPSs possess an identical core oligosaccharide.
13C NMR, 1H NMR, methylation, NMR-2D, SDS-PAGE, ELISA, ESI-MS, ESI-MS/MS, GC, Western blotting, de-O-acetylation, serological methods, conjugation
variant NMR chemical shifts of the de-O-acetylated core oligosaccharide residues -4)aDGalpN 1H: 5.23 3.60 4.19 4.08 4.50 3.61-3.70, 13C: 96.5 51.7 66.9 76.9 72.4 60.3, and -4)aDGlcpNAc 1H: 4.88 3.92 4.04 3.75 4.28 3.79-3.88, 13C: 99.2 54.6 69.7 79.5 71.5 60.5.
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7 C8
5,3,3,2,2 aXLDmanHepp 103.1 70.8 71.3 67.0 73.4 69.8 64.0
5,3,3,2 aXDDmanHepp 95.5 80.6 70.6 68.3 74.3 72.4 62.9
5,3,3,4,4,4 bDGalp 103.7 71.8 73.4 69.5 76.3 61.9
5,3,3,4,4,2 Ac 175.4 22.7
5,3,3,4,4 aDGlcpN 98.8 54.4 69.8 79.6 71.5 ?
5,3,3,4,6 Ac 174.5 21.0
5,3,3,4 aDGalpN 97.3 51.7 66.3 77.6 69.7 62.3
5,3,3 aDGalpA 99.0 72.7 67.3 80.1 72.2 176.0
5,3,6,0 xXEtN 63.0 41.0
5,3,6 P
5,3,7 aXLDmanHepp 100.4 70.9 69.5 67.1 72.4 69.8 63.8
5,3 aXLDmanHepp 101.0 71.6 79.7 66.1 73.3 74.1 68.2
5,4 bDGlcp 103.3 74.7 76.3 70.4 76.9 62.0
5 aXLDmanHepp 101.6 70.6 74.4 76.5 72.1 70.5 64.3
8 bLArap4N 99.1 68.8 66.5 52.8 58.8
aXKdop ? ? 35.4 66.8 75.7 71.7 68.2 69.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7 H8
5,3,3,2,2 aXLDmanHepp 5.02 4.02 3.82 3.83 3.66 4.01 3.67-3.72
5,3,3,2 aXDDmanHepp 5.27 3.91 4.00 3.75 3.89 3.95 3.65-3.81
5,3,3,4,4,4 bDGalp 4.48 3.53 3.64 3.91 3.71 3.72-3.75
5,3,3,4,4,2 Ac - 2.06
5,3,3,4,4 aDGlcpN 4.95 3.93 4.05 3.78 4.29 ?
5,3,3,4,6 Ac - 2.10
5,3,3,4 aDGalpN 5.22 3.62 4.18 4.11 4.73 4.04-4.21
5,3,3 aDGalpA 5.45 4.04 4.21 4.43 4.46 -
5,3,6,0 xXEtN 4.16 3.28
5,3,6 P
5,3,7 aXLDmanHepp 4.93 3.96 3.89 3.82 3.65 4.02 3.69
5,3 aXLDmanHepp 4.89 3.98 4.01 3.99 3.81 4.69 3.88
5,4 bDGlcp 4.58 3.30 3.49 3.37 3.37 3.74-3.86
5 aXLDmanHepp 5.07 4.06 3.96 4.21 4.11 3.97 3.72
8 bLArap4N 4.98 3.72 4.14 3.68 3.73-4.10
aXKdop - - 1.80-2.19 4.09 4.16 4.03 3.74 3.60-3.92
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7 C8/H8
5,3,3,2,2 aXLDmanHepp 103.1/5.02 70.8/4.02 71.3/3.82 67.0/3.83 73.4/3.66 69.8/4.01 64.0/3.67-3.72
5,3,3,2 aXDDmanHepp 95.5/5.27 80.6/3.91 70.6/4.00 68.3/3.75 74.3/3.89 72.4/3.95 62.9/3.65-3.81
5,3,3,4,4,4 bDGalp 103.7/4.48 71.8/3.53 73.4/3.64 69.5/3.91 76.3/3.71 61.9/3.72-3.75
5,3,3,4,4,2 Ac 22.7/2.06
5,3,3,4,4 aDGlcpN 98.8/4.95 54.4/3.93 69.8/4.05 79.6/3.78 71.5/4.29 ?/?
5,3,3,4,6 Ac 21.0/2.10
5,3,3,4 aDGalpN 97.3/5.22 51.7/3.62 66.3/4.18 77.6/4.11 69.7/4.73 62.3/4.04-4.21
5,3,3 aDGalpA 99.0/5.45 72.7/4.04 67.3/4.21 80.1/4.43 72.2/4.46
5,3,6,0 xXEtN 63.0/4.16 41.0/3.28
5,3,6 P
5,3,7 aXLDmanHepp 100.4/4.93 70.9/3.96 69.5/3.89 67.1/3.82 72.4/3.65 69.8/4.02 63.8/3.69
5,3 aXLDmanHepp 101.0/4.89 71.6/3.98 79.7/4.01 66.1/3.99 73.3/3.81 74.1/4.69 68.2/3.88
5,4 bDGlcp 103.3/4.58 74.7/3.30 76.3/3.49 70.4/3.37 76.9/3.37 62.0/3.74-3.86
5 aXLDmanHepp 101.6/5.07 70.6/4.06 74.4/3.96 76.5/4.21 72.1/4.11 70.5/3.97 64.3/3.72
8 bLArap4N 99.1/4.98 68.8/3.72 66.5/4.14 52.8/3.68 58.8/3.73-4.10
aXKdop 35.4/1.80-2.19 66.8/4.09 75.7/4.16 71.7/4.03 68.2/3.74 69.4/3.60-3.92
report error
Found 1 record.
Displayed record 1
]; wound infections [ICD11: NF0A.3 
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