Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea (escherichiosis) [ICD11:
ME05.1 
, ICD11:
SA55 , ICD11:
XN6P4 ];
enterocolitis [ICD11:
1A40.Z ];
hemorrhagic colitis (HC) [ICD11:
1A40.0 ];
hemolytic-uremic syndrome (HUS) [ICD11:
3A21.2 ];
infection due to Escherichia coli [ICD11:
XN6P4 ]
The structure was elucidated in this paperPublication DOI: 10.1007/s11172-018-2340-zJournal NLM ID: 100912060Publisher: New York: Consultants Bureau
Correspondence: yknirel
gmail.com
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Structure of the O-polysaccharide (O-antigen) of Escherichia coli O60 was studied by sugar analysis, partial solvolysis with CF3CO2H, and 1D and 2D 1H and 13C NMR spectroscopy. The O-polysaccharide was found to consist of D-galactose and L-rhamnose. The structure of its branched tetrasaccharide repeating unit was established, which is unique among known bacterial polysaccharide structures.
Lipopolysaccharide, O-antigen, Escherichia, Escherichia coli, bacterial polysaccharide structure, solvolysis
Structure type: polymer chemical repeating unit
Location inside paper: p.2134, fig.3
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_133754,IEDB_136044,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,IEDB_225177,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ESI-MS, acid hydrolysis, mild acid hydrolysis, GC, GPC, solvolysis with trifluoroacetic acid
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G82615LC
Show glycosyltransferases
NMR conditions: in D2O at 293 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,2 aLRhap 100.1 79.5 70.7 73.7 70.9 17.9
4,3 aDGalp 102.2 69.7 70.8 70.7 73.3 62.8
4 aLRhap 101.6 76.3 78.8 73.3 70.9 17.6
bDGalp 104.3 71.6 72.9 78.9 76.7 62.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,2 aLRhap 5.28 4.21 3.84 3.48 3.86 1.34
4,3 aDGalp 5.31 3.86 3.83 3.99 3.96 3.73-3.80
4 aLRhap 4.84 4.27 4.02 3.71 4.17 1.27
bDGalp 4.48 3.58 3.78 4.01 3.76 3.76-3.76
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,2 aLRhap 100.1/5.28 79.5/4.21 70.7/3.84 73.7/3.48 70.9/3.86 17.9/1.34
4,3 aDGalp 102.2/5.31 69.7/3.86 70.8/3.83 70.7/3.99 73.3/3.96 62.8/3.73-3.80
4 aLRhap 101.6/4.84 76.3/4.27 78.8/4.02 73.3/3.71 70.9/4.17 17.6/1.27
bDGalp 104.3/4.48 71.6/3.58 72.9/3.78 78.9/4.01 76.7/3.76 62.0/3.76-3.76
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,2 | aLRhap | 5.28 | 4.21 | 3.84 | 3.48 | 3.86 | 1.34 |
| 4,3 | aDGalp | 5.31 | 3.86 | 3.83 | 3.99 | 3.96 | 3.73
3.80 |
| 4 | aLRhap | 4.84 | 4.27 | 4.02 | 3.71 | 4.17 | 1.27 |
| | bDGalp | 4.48 | 3.58 | 3.78 | 4.01 | 3.76 | 3.76
3.76 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,2 | aLRhap | 100.1 | 79.5 | 70.7 | 73.7 | 70.9 | 17.9 |
| 4,3 | aDGalp | 102.2 | 69.7 | 70.8 | 70.7 | 73.3 | 62.8 |
| 4 | aLRhap | 101.6 | 76.3 | 78.8 | 73.3 | 70.9 | 17.6 |
| | bDGalp | 104.3 | 71.6 | 72.9 | 78.9 | 76.7 | 62.0 |
|
There is only one chemically distinct structure:
Found 
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