Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperPublication DOI: 10.1007/s11172-018-2310-5Journal NLM ID: 100912060Publisher: New York: Consultants Bureau
Correspondence: andreivperepelov
gmail.com
Institutions: N. D. Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussian Federation
The structure of O-polysaccharide (O-antigen) of Escherichia coli O95 was established using the analysis of deacylated lipopolysaccharide of E. coli O95 by 2D 1H and 13C NMR spectroscopy. It was found to consist of disaccharide repeating units containing one of each 3-substituted residues of d-fucose and d-threo-pent-2-ulose (xylulose)
Lipopolysaccharide, Escherichia coli, O-polysaccharide, O-antigen gene cluster, structure of bacterial polysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: p.1932, table 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115015,IEDB_142489,IEDB_149135,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, GPC, alkaline deacylation, optical rotation measurement, bioinformatic analysis
Related record ID(s): 12757
NCBI Taxonomy refs (TaxIDs): 562
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3 bDXulf 62.0 107.2 83.7 75.4 72.0
aDFucp 100.2 67.8 72.1 73.5 68.3 16.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3 bDXulf 3.71-3.76 - 4.29 4.63 3.70-4.22
aDFucp 5.21 3.85 4.14 3.77 4.17 1.17
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3 bDXulf 62.0/3.71-3.76 83.7/4.29 75.4/4.63 72.0/3.70-4.22
aDFucp 100.2/5.21 67.8/3.85 72.1/4.14 73.5/3.77 68.3/4.17 16.7/1.17
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3 | bDXulf | 3.71
3.76 |
| 4.29 | 4.63 | 3.70
4.22 | |
| | aDFucp | 5.21 | 3.85 | 4.14 | 3.77 | 4.17 | 1.17 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3 | bDXulf | 62.0 | 107.2 | 83.7 | 75.4 | 72.0 | |
| | aDFucp | 100.2 | 67.8 | 72.1 | 73.5 | 68.3 | 16.7 |
|
There is only one chemically distinct structure:
Found 
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