Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Host organism: Homo sapiens
Associated disease: hemorrhagic pneumonia [ICD11:
CA40 
]
The structure was elucidated in this paperPublication DOI: 10.1038/s41598-018-29864-yJournal NLM ID: 101563288Publisher: London: Nature Publishing Group
Correspondence: pasciak
iitd.pan.wroc.pl
Institutions: Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wroclaw, Poland, Department of Medical Biochemistry, Wroclaw Medical University, Chaіubiсskiego 10, 50-368, Wroclaw, Poland, Department of Pathology, Wrocław Medical University, Marcinkowskiego 1, 50-368, Wrocław, Poland, USI, Unit of Nanostructural Bio-Interactions, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wroclaw, Poland, Wrocław Research Center EIT+, Stablowicka 147, 54-066, Wrocław, Poland. Correspondence and requests for materials should be addressed to Mariola Pasciak
Tsukamurella pulmonis is an opportunistic actinomycetal pathogen associated with a variety of rarely diagnosed human infections. In clinical cases of infection, T. pulmonis usually accompanies other bacterial pathogens. Because of these mixed infections, a robust diagnostic assay is important. The bacteria cell surface polysaccharides are considered not only useful targets for diagnostics but also intriguing subjects for analysis of the interactions that regulate the host response in general. Here, the structure of the polysaccharide component of the T. pulmonis cell wall was established. Sugar and methylation analysis and 2D-NMR techniques revealed that its polysaccharide belongs to the class of arabinomannan composed of branched tetrasaccharide repeating units, with addition of linear →6)-α-D-Manp-(1→ mannan. Rabbit polyclonal sera against T. pulmonis and T. paurometabola bacterial cells revealed cross reactivity between their antigens. Tissue samples from mice infected with T. pulmonis revealed liver abscesses and pathologic granules located intracellularly when immunohistochemically stained with monoclonal antibodies raised against T. pulmonis polysaccharide. Ultrastructural studies revealed that these granules contain T. pulmonis cells. These observations indicate that T. pulmonis is a pathogenic species capable of spreading within the organism, presumably through the blood.
NMR, monoclonal antibodies, cross-reactivity, neutral polysaccharide, Tsukamurella pulmonis
Structure type: homopolymer
Location inside paper: fig.2
Trivial name: LAM, mannan, galactomannan, cell wall heteromannan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, N-glycan, cell surface polysaccharide, mannan, D-mannan, galactomannan
Contained glycoepitopes: IEDB_130701,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, ELISA, MALDI-TOF MS, serological methods, electron microscopy, immunochemical methods, hydrophobicity test
Related record ID(s): 12777
NCBI Taxonomy refs (TaxIDs): 47312Reference(s) to other database(s): GTC:G55317BB, GlycomeDB:
6776, CCSD:
46066, CBank-STR:6345
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDManp 101.8 72.3 72.9 69.1 75.6 67.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDManp 4.88 3.98 3.80 3.69 3.76 3.76-3.92
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDManp 101.8/4.88 72.3/3.98 72.9/3.80 69.1/3.69 75.6/3.76 67.9/3.76-3.92
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDManp | 4.88 | 3.98 | 3.80 | 3.69 | 3.76 | 3.76
3.92 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDManp | 101.8 | 72.3 | 72.9 | 69.1 | 75.6 | 67.9 |
|
There is only one chemically distinct structure:
Found 
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