Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperPublication DOI: 10.1002/(SICI)1097-458X(199801)Journal NLM ID: 9882600Publisher: Wiley Heyden
Institutions: Central Institute of Medicinal and Aromatic Plants, Lucknow 226 015, India, Department of Chemistry and Biochemistry, University of Maryland, Baltimore County Campus, Baltimore, Maryland 21228, USA, Mycobacteriology Research Laboratory, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin 53705, Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA
The hexamethyl derivative of lipopolysaccharide (LPS), obtained by diazomethane methylation of LPS extracted from a deep mutant of Escherichia coli D31m4, followed by high-performance liquid chromatographic purification, was subjected to homo- and heteronuclear two-dimensional NMR spectroscopy (DQF-COSY, HOHAHA, NOESY, HMQC and HMBC) to achieve 1H and 13C NMR assignments, particularly of the sugar backbone. These studies confirmed two each of glucosamine (GlcN), 3-deoxy-D-manno-octulosonic acid (Kdo), phosphate, b-hydroxymyristate and b-acyloxymyristate and the structure of the tetrasaccharide as aXKdop(2-4)aXKdoP(2-6)bDGlcpN(1-6)aDGlcpN bisphosphorylated at the 1 and 4' positions.
Lipopolysaccharide, NMR, LPS, structure, core, Escherichia, Escherichia coli, mutant, rough, 13C NMR, R-type LPS, assignment, 1H NMR, assignments, rough LPS
Structure type: oligomer
Location inside paper: p. 2
Aglycon: lipid A
Trivial name: outer core region
Compound class: core oligosaccharide, LOS, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130659
Methods: NMR-2D, HPLC
Related record ID(s): 829, 852, 875, 924, 6252, 6306, 6431, 7184, 8403, 10584, 10590, 23706, 24940
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G36348JQ, GlycomeDB:
5576
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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