Vann WF, Jann K Structure and serological specificity of the K13-antigenic polysaccharide (K13 antigen) of urinary tract-infective Escherichia coli Infection and Immunity25 (1979)
85-92
/Variants 0/-+
|
-3)-Ribf-(1-7)-Kdo-(2-
/Variants 0/ is:
?%Ac-5)-
OR (exclusively)
?%Ac-4)-
The structure was elucidated in this paper NCBI PubMed ID:90016 Journal NLM ID:0246127 Publisher: American Society for Microbiology Institutions: Max-Planck-Institut für Immunbiologie, Freiberg, West Germany
The primary structure of the K13-antigenic polysaccharide (K13 antigen) of Escherichia coli O6:K13:H1 was elucidated by composition, periodate oxidation, Smith degradation, and methylation analysis. The polysaccharide consists of a repeating sequence of 3-linked ribofuranose and 7-linked 3-deoxymannooctulosonic acid (KDO). About 50% of the KDO residues are O-acetylated at position 4 or 5. Measurement of the optical rotary dispersion indicated that in aqueous solution the K13 polysaccharide assumes a secondary structure in which the carboxyl groups of KDO are engaged. The serological specificity of the K13 polysaccharide is expressed through KDO and its O-acetyl substituent, the ribose unit being antigenically silent. There are two populations of anti-K13 antibodies one directed against the charged region of the KDO and the other against the O-acetyl groups.
Structure type: polymer chemical repeating unit Location inside paper: p. 91 Compound class: CPS, K-antigen Contained glycoepitopes:IEDB_130650,IEDB_149136
Methods: methylation, periodate oxidation, Smith degradation, composition analysis, serological methods Comments, role: total degree of Kdo acetylation is 50%
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