Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 8112871Journal NLM ID: 0246127Publisher: American Society for Microbiology
Institutions: Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan
The cell wall mannans of two Candida albicans serotype A strains, NIH A-207 and J-1012 (abbreviated as A and J strains, respectively), cultured in yeast extract-Sabouraud liquid medium at pH 2.0, contained neither a phosphate group nor a β-1,2-linked mannopyranose unit (H. Kobayashi, P. Giummelly, S. Takahashi, M. Ishida, J. Sato, M. Takaku, Y. Nishidate, N. Shibata, Y. Okawa, and S. Suzuki, Biochem. Biophys. Res. Commun. 175:1003-1009, 1991). In this study, the mannans obtained from A and J strains grown in pH 2.0 medium (abbreviated as mannans A2 and J2, respectively) exhibited quite different reactivities against rabbit anti-C. albicans and anti-Saccharomyces cerevisiae sera compared with those of mannans from the corresponding strains cultured in conventional medium at pH 5.9 (abbreviated as mannans A and J, respectively). Namely, mannans A2 and J2 lost reactivity against the former serum but reacted with the latter serum to a higher extent than mannans A and J. In order to account for these difference in more detail, mannans A2 and J2 were subjected to acetolysis. Elution profiles of the acetolysates were completely different from those of acetolysates obtained from mannans A and J reported in our previous papers. The 1H nuclear magnetic resonance spectra of the oligosaccharides from mannans A2 and J2 obtained by this procedure indicate that the side chains are composed of α-linked mannopyranose units densely linked to the α-1,6-linked backbone. The long side chains containing one α-1,3-linked mannopyranose unit are markedly increased.
Structure type: oligomer
Location inside paper: Table 3, M2a
Compound class: mannan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 1H NMR, acetolysis, hot-water extraction, short-term precipitation, quantitative precipitin assay
Comments, role: Parent molecule: mannan; degradation product
Related record ID(s): 130516, 130517, 130519, 130520, 130521, 130522, 130523, 130524, 130525, 136630
NCBI Taxonomy refs (TaxIDs): 5476Reference(s) to other database(s): GTC:G53402KW, GlycomeDB:
278, CCSD:
32606, CBank-STR:2845
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error