Taxonomic group: bacteria / Actinobacteria
(Phylum: Actinobacteria)
Host organism: Homo sapiens
Associated disease: tuberculosis [ICD11:
XN1N2 
];
infection due to Mycobacterium tuberculosis [ICD11:
XN1N2 ]
NCBI PubMed ID: 29761597Publication DOI: 10.1002/pro.3443Journal NLM ID: 9211750Publisher: Woodbury, NY: Cold Spring Harbor Laboratory Press for the Protein Society
Correspondence: Hazel_Holden
biochem.wisc.edu
Institutions: Human Health Therapeutics, National Research Council Canada, 100 Sussex Drive, Ottawa, ON, Canada, K1A OR6, Department of Biochemistry, University of Wisconsin, Madison, WI 53706
Recent studies have demonstrated that the O-antigens of some pathogenic bacteria such as Brucella abortus, Francisella tularensis, and Campylobacter jejuni contain quite unusual N-formylated sugars (3-formamido-3,6-dideoxy-d-glucose or 4-formamido-4,6-dideoxy-d-glucose). Typically, four enzymes are required for the formation of such sugars: a thymidylyltransferase, a 4,6-dehydratase, a pyridoxal 5'-phosphate or PLP-dependent aminotransferase, and an N-formyltransferase. To date, there have been no published reports of N-formylated sugars associated with Mycobacterium tuberculosis. A recent investigation from our laboratories, however, has demonstrated that one gene product from M. tuberculosis, Rv3404c, functions as a sugar N-formyltransferase. Given that M. tuberculosis produces l-rhamnose, both a thymidylyltransferase (Rv0334) and a 4,6-dehydratase (Rv3464) required for its formation have been identified. Thus, there is one remaining enzyme needed for the production of an N-formylated sugar in M. tuberculosis, namely a PLP-dependent aminotransferase. Here we demonstrate that the M. tuberculosis rv3402c gene encodes such an enzyme. Our data prove that M. tuberculosis contains all of the enzymatic activities required for the formation of dTDP-4-formamido-4,6-dideoxy-d-glucose. Indeed, the rv3402c gene product likely contributes to virulence or persistence during infection, though its temporal expression and location remain to be determined. This article is protected by copyright. All rights reserved
6-dideoxy-d-glucose, Mycobacterium tuberculosis, aminotransferase, dTDP-4-amino-4, pyridoxal 5'-phosphate, Rv3402c
Structure type: monomer
Location inside paper: p.1493, scheme 1, dTDP-4-formamido-4,6-dideoxy-d-glucose
Trivial name: dTDP-4,6-dideoxy-4-formamido-D-glucose, dTDP-4-formamido-4,6-dideoxy-d-glucose
Contained glycoepitopes: IEDB_138113,IEDB_196259
Methods: 13C NMR, 1H NMR, DNA techniques, 31P NMR, ESI-MS, HPLC, enzyme assay, cloning, gene expression
Enzymes that release or process the structure: 4-N-formyltransferase
Biosynthesis and genetic data: Rv3404c
Related record ID(s): 12875
NCBI Taxonomy refs (TaxIDs): 1773
Show glycosyltransferases
There is only one chemically distinct structure:
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