Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
infection due to Campylobacter jejuni [ICD11:
XN4Q5 ]
The structure was elucidated in this paperNCBI PubMed ID: 30049709Publication DOI: 10.1194/jlr.M085860Journal NLM ID: 0376606Publisher: ASBMB
Correspondence: gary.jarvis
ucsf.edu
Institutions: Infection, Immunity and Inflammation Programme, University College London Great Ormond Street Institute of Child Health, London, United Kingdom, Department of Laboratory Medicine University of California, San Francisco, CA, Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, Center for Genome-Enabled Biology and Medicine, School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK, Department of Paediatric Gastroenterology, Royal Hospital for Children, Glasgow, United Kingdom, Department of Infectious Diseases Aalborg University Hospital, Aalborg, Denmark, Department of Clinical Microbiology, Aalborg University Hospital, Aalborg, Denmark, St George and Sutherland Clinical School, University of New South Wales, Sydney, Australia, Center for Immunochemistry, Veterans Affairs Medical Center, San Francisco, CA
The pathogenicity of Campylobacter concisus, increasingly found in the human gastrointestinal (GI) tract, is unclear. Some studies indicate that its role in GI conditions has been underestimated, whereas others suggest that the organism has a commensal-like phenotype. For the enteropathogen C. jejuni, the lipooligosaccharide (LOS) is a main driver of virulence. We investigated the LOS structure of four C. concisus clinical isolates and correlated the inflammatory potential of each isolate with bacterial virulence. Mass spectrometric analyses of lipid A revealed a novel hexa-acylated diglucosamine moiety with two or three phosphoryl substituents. Molecular and fragment ion analysis indicated that the oligosaccharide portion of the LOS had only a single phosphate and lacked phosphoethanolamine and sialic acid substitution, which are hallmarks of the C. jejuni LOS. Consistent with our structural findings, C. concisus LOS and live bacteria induced less TNF-α secretion in human monocytes than did C. jejuni Furthermore, the C. concisus bacteria were less virulent than C. jejuni in a Galleria mellonella infection model. The correlation of the novel lipid A structure, decreased phosphorylation, and lack of sialylation along with reduced inflammatory potential and virulence support the significance of the LOS as a determinant in the relative pathogenicity of C. concisus.
Lipooligosaccharide, lipid A, mass spectrometry, inflammation, phosphorylation, glycolipids, cytokines, Monocytes, Campylobacter concisus, toll like receptors
Structure type: oligomer
Location inside paper: fig.4 C. jejuni 11168H
Compound class: lipid A
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_141181
Methods: PCR, SDS-PAGE, MS/MS, MALDI-TOF MS, de-O-acylation with hydrazine, HF treatment, statistical analysis, cytokines tumor necrosis factor alpha (TNF-a) assay, function analysis of gene clusters
Comments, role: variant of lipid A, as the lipid A disaccharide of C. jejuni is comprised of either 2-amino-2-deoxy-D-glucose (GlcN) or 2,3-diamino-2,3-dideoxy-D-glucose (GlcN3N).
Related record ID(s): 12876
NCBI Taxonomy refs (TaxIDs): 197
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There is only one chemically distinct structure:
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