Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 7844147Journal NLM ID: 0375356Publisher: New York: Rockefeller University Press
Institutions: Department of Biological Sciences, Hunter College of the City University of New York, New York 10021
The cell adhesion protein α-agglutinin is bound to the outer surface of the Saccharomyces cerevisiae cell wall and mediates cell-cell contact in mating. α-Agglutinin is modified by addition of a glycosyl phosphatidylinositol (GPI) anchor as it traverses the secretory pathway. The presence of a GPI anchor is essential for cross-linking into the wall, but the fatty acid and inositol components of the anchor are lost before cell wall association (Lu, C.-F., J. Kurjan, and P. N. Lipke, 1994. A pathway for cell wall anchorage of Saccharomyces cerevisiae α-agglutinin. Mol. Cell. Biol. 14:4825-4833). Cell wall association of α-agglutinin was accompanied by an increase in size and a gain in reactivity to antibodies directed against β-1,6-glucan. Several kre mutants, which have defects in synthesis of cell wall β-1,6-glucan, had reduced molecular size of cell wall α-agglutinin. These findings demonstrate that the cell wall form of α-agglutinin is covalently associated with β-1,6-glucan. The α-agglutinin biosynthetic precursors did not react with antibody to β-1,6-glucan, and the sizes of these forms were unaffected in kre mutants. A COOH-terminal truncated form of α-agglutinin, which is not GPI anchored and is secreted into the medium, did not react with the anti-β-1,6-glucan. We propose that extracellular cross-linkage to β-1,6-glucan mediates covalent association of α-agglutinin with the cell wall in a manner that is dependent on prior addition of a GPI anchor to α-agglutinin.
Structure type: homopolymer
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Related record ID(s): 47842, 47843, 48303, 48366, 48412, 48423, 48443, 49291, 101372, 124916, 143682, 149870, 149873
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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