Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Brucella melitensis [ICD11:
XN7ZW 
]
Journal NLM ID: 0376514WWW link: http://pac.iupac.org/publications/pac/pdf/1989/pdf/6107x1171.pdfPublisher: Oxford: Blackwell Scientific Publications
Institutions: Division of Biological Science, National Research Council of Canada, Ottawa, Ontario, K1A OR6, Canada
Synthetic oligosaccharides and polysaccharides of known structure have been covalently attached to carrier protein and enzymes for use in a strategy to generate and select monoclonal antibodies with well defined carbohydrate binding profiles. Direct competitive binding assays utilizing antibody bound to a solid phase and saccharide - enzyme conjugates provided convenient and reliable measurement of specificity . Protocols were developed to prepare these carbohydrate - enzyme conjugates. Antibodies specific for the Brucella A and M polysaccharide/antigen were selected and characterized by these techniques. The crystal structure of an Fab fragment derived from one antibody has been solved at 2.7A resolution. Preliminary crystal structure data in conjunction with the amino acid sequence of light and Fd polypeptide chains implicate tyrosine, arginine, asparagine, glutamine and aspartic acid as the residues that contact antigen. A second a antibody - antigen system involving the Shigella flexneri Y antigen has been studied by a similar approach, although in this case two distinct antibodies have been subjected to detailed binding studies with oligosaccharide fragments of the polysaccharide repeating unit and specifically modified derivatives. These data support the contention that the principal polar interactions between an oligosaccharide and its antibody involve a small group of hydroxyl residues and that specifically deoxygenated oligosaccharide inhibitors can have association constants significantly higher than the natural oligosaccharide.
Structure type: polymer chemical repeating unit
Trivial name: M antigen
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130647,IEDB_131172,IEDB_134279,IEDB_134280,IEDB_134281,IEDB_1397515,IEDB_2116320,IEDB_434544,IEDB_434545,IEDB_434546
Related record ID(s): 107325, 108579, 130218, 139863, 148072, 148073, 148074
NCBI Taxonomy refs (TaxIDs): 29459Reference(s) to other database(s): GTC:G53253RW, GlycomeDB:
3021
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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