Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 7725794Publication DOI: 10.1002/yea.320101208Journal NLM ID: 8607637Publisher: Chichester, Wiley
Institutions: Department of Plant Biology, University of Groningen, Haren, The Netherlands
Pulse-chase experiments with [14C]glucose demonstrated that in the cell wall of wild-type Saccharomyces cerevisiae alkali-soluble (1-3)-β-glucan serves as a precursor for alkali-insoluble (1-3)-β-glucan. The following observations support the notion that the insolubilization of the glucan is caused by linkage to chitin: (i) degradation of chitin by chitinase completely dissolved the glucan, and (ii) disruption of the gene for chitin synthase 3 prevented the formation of alkali-insoluble glucan. These cells, unable to form a glucan-chitin complex, were highly vulnerable to hypo-osmotic shock indicating that the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall. Conversion of alkali-soluble glucan into alkali-insoluble glucan occurred both early and late during budding and also in the ts-mutant cdc24-1 in the absence of bud formation.
Structure type: homopolymer ; n is large
Trivial name: chitin
Compound class: chitin
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Related record ID(s): 41640, 42301, 42350, 42638, 49501, 49523, 49564, 49568, 50015, 50302, 50305, 50306, 50309, 50312, 50313, 50316, 50318, 111874, 121703, 131817, 139821, 143656, 147987, 149875
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G97099AY, CCSD:
50510, CBank-STR:4329
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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