Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Organ / tissue: cell wall,
biofilm
NCBI PubMed ID: 38350588Publication DOI: 10.1016/j.ijbiomac.2024.130121Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Dos Santos Ré AC <ana3.santos
alumni.usp.br>; Cury JA <jcury
fop.unicamp.br>; Sassaki GL <sassaki
ufpr.br>; Aires CP <airescp
fcfrp.usp.br>
Institutions: Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil, Department of Biosciences, Piracicaba Dental School, UNICAMP, Piracicaba, Brazil, Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil
This study identified a rhamnose-containing cell wall polysaccharide (RhaCWP) in an alkaline extract prepared to analyze intracellular polysaccharides (IPS) from Streptococcus mutans biofilm. IPS was an 1,4-α-D-glucan with branchpoints introduced by 1,6-α-glucan while RhaCWP presented 1,2-α-L-and 1,3-α-L rhamnose backbone and side chains connected by 1,2-α-D-glucans, as identified by nuclear magnetic resonance (NMR) spectroscopy and methylation analyses. The MW of IPS and RhaCWP was 11,298 Da, as determined by diffusion-ordered NMR spectroscopy. Therefore, this study analyzed the chemical structure of RhaCWP and IPS from biofilm in a single fraction prepared via a convenient hot-alkali extraction method. This method could be a feasible approach to obtain such molecules and improve the comprehension of the structure-function relationships in polymers from S. mutans in future studies
Biofilm, rhamnoglucan, dental caries, intracellular polysaccharide
Structure type: homopolymer
Location inside paper: pg. 4, Table 2, IPS
Trivial name: methyl glucose lipopolysaccharide, glucan, maltosaccharide, α-1,4-D-glucan, amylose, α-glucan, glycogen backbone, α-(1,4)-glucan, starch, α-(1-4)-glucan, starch, glycogen
Compound class: CPS, EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, methyl glucose lipopolysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, acid hydrolysis, HPLC, HPSEC, extraction, acetylation, methylation analysis, reduction, precipitation, derivatization, evaporation, sonication, centrifugation
Related record ID(s): 15661
NCBI Taxonomy refs (TaxIDs): 1309Reference(s) to other database(s): GTC:G05740LL, GlycomeDB:
12100, CCSD:
4943, CBank-STR:819
Show glycosyltransferases
NMR conditions: in D2O / TSP at 343(C), 298(H) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 100.2 72.2 73.7 78.0 71.9 61.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 5.38 3.65 3.97 3.65 3.84 3.82-3.88
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 100.2/5.38 72.2/3.65 73.7/3.97 78.0/3.65 71.9/3.84 61.1/3.82-3.88
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 5.38 | 3.65 | 3.97 | 3.65 | 3.84 | 3.82
3.88 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 100.2 | 72.2 | 73.7 | 78.0 | 71.9 | 61.1 |
|
There is only one chemically distinct structure:
Found 
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