Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 16936038Publication DOI: 10.1128/JB.00783-06Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: rickp
usuhs.mil
Institutions: Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799, and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8816
The carbohydrate component of the enterobacterial common antigen (ECA) of Escherichia coli K-12 occurs primarily as a water-soluble cyclic polysaccharide located in the periplasm (ECACYC) and as a phosphoglyceride-linked linear polysaccharide located on the cell surface (ECAPG). The polysaccharides of both forms are comprised of the amino sugars, N-acetyl-D-glucosamine (GlcNAc), N-acetyl-D-mannosaminuronic acid (ManNAcA) and 4-acetamido-4,6-dideoxy-D-galactose (Fuc4NAc). These amino sugars are linked to one another to form trisaccharide repeat units with the structure, →3-α-D-Fuc4NAc-(1→4)-β-D-ManNAcA-(1→4)-α-D-GlcNAc-(1→. The hydroxyl group in the 6-position of the GlcNAc residues of both ECACYC and ECAPG are non-stoichiometrically esterified with acetyl groups. Random transposon insertion mutagenesis of E. coli K-12 resulted in the generation of a mutant defective in the incorporation of O-acetyl groups into both ECACYC and ECAPG. This defect was found to be due to insertion of the transposon into the yiaH locus, a putative gene of unknown function located at 80.26 min on the E. coli chromosomal map. Bioinformatic analyses of the predicted yiaH gene product indicate that it is an integral inner membrane protein that is a member of an acyltransferase family of enzymes found in a wide variety of organisms. The results of biochemical and genetic experiments presented here strongly support the conclusion that yiaH encodes the O-acetyltransferase responsible for the incorporation of O-acetyl groups into both ECACYC and ECAPG. Accordingly, we propose that this gene be designated wecH.
structure, gene, enterobacterial common antigen, ECA, O-acetylation, acyltransferase, Escherichia coli K-12
Structure type: polymer chemical repeating unit
Location inside paper: abstract
Compound class: enterobacterial common antigen
Contained glycoepitopes: IEDB_141807,IEDB_151531
Methods: NMR, MALDI-TOF MS
Biosynthesis and genetic data: biochemical data, genetic data
NCBI Taxonomy refs (TaxIDs): 83333Reference(s) to other database(s): GTC:G11367GJ
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error